Description

2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride is a specialized tetrazolium salt widely used as a vital reagent in biochemical and analytical applications. Its primary value lies in its role as a redox indicator and a precursor for formazan dye formation, which is critical for cell viability and metabolic activity assays. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Microbiological Testing: Employed as an indicator for microbial growth and metabolic activity in environmental and clinical samples.
• Enzyme Activity Studies: Serves as an electron acceptor in dehydrogenase enzyme assays, facilitating colorimetric detection.
• Pharmaceutical Research: Utilized in high-throughput screening (HTS) to evaluate the effects of drug candidates on cell proliferation.
• Biochemical Reagent: Acts as a sensitive chromogenic agent in various diagnostic and research kits.
• Material Science Research: Applied in studies involving redox-active polymers and coatings.

Basic Information

Product Name	2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride
CAS No.	16755-66-1
Molecular Formula	C26H21ClN4
Molecular Weight	424.92 g/mol
Synonyms	TPTZ Chloride; p-Tolyl Tetrazolium Chloride; 5-(4-Methylphenyl)-2,3-diphenyl-2H-tetrazol-3-ium Chloride; Tetrazolium Red Chloride (related); MTT Tetrazolium Salt Analog; TTC Derivative; INT Derivative; NSC 406130
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-(p-Tolyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under low-light conditions to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility Test	Clear, yellow solution in DMSO or ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.