Description

5-Bromo-3-Indoxyl Phosphate, Disodium Salt is a high-purity chromogenic substrate widely used in enzymatic detection systems. Its primary value lies in its ability to produce an insoluble, intensely colored indigo dye upon enzymatic hydrolysis, enabling sensitive and precise visualization. This compound is essential for researchers and quality control laboratories in the biotechnology, diagnostic, and life science sectors.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): As a key substrate for alkaline phosphatase (AP) in colorimetric immunoassays for protein and antibody detection.
• Western Blotting: For the chromogenic detection of proteins and nucleic acids following transfer to membranes.
• Histochemistry & In Situ Hybridization: Used to localize specific enzymes or genetic material in tissue sections with high spatial resolution.
• Microbiology & Cell Biology: Employed in assays to detect and quantify bacterial or cellular enzymatic activity, such as reporter gene assays.
• Diagnostic Kit Manufacturing: A critical raw material in the production of clinical diagnostic kits for various diseases and biomarkers.
• Research & Development: For developing and optimizing new biochemical assays and detection methodologies in academic and industrial R&D.

Basic Information

Product Name	5-Bromo-3-Indoxyl Phosphate, Disodium Salt
CAS No.	16036-59-2
Molecular Formula	C8H6BrNO4P·2Na
Molecular Weight	349.99 g/mol
Synonyms	5-Bromo-3-indolyl phosphate disodium salt; BCIP, Disodium Salt; 5-Bromo-4-chloro-3-indolyl phosphate (common abbreviation BCIP); 5-Br-3-Indoxyl phosphate disodium; 3-Indoxyl phosphate, 5-bromo-, disodium salt; BCIP disodium; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate disodium salt
EINECS	240-177-9

Quality Control

Our 5-Bromo-3-Indoxyl Phosphate, Disodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in water
Enzymatic Activity	Suitable for use (Performance Test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.