Description

5-Bromo-4-Chloro-3-Indolyl-β-D-Glucoside is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its ability to produce an insoluble, intensely colored indigo dye upon enzymatic hydrolysis, making it an essential reagent for visualizing and quantifying β-glucuronidase activity. This compound is primarily needed by manufacturers of culture media, research institutions, and diagnostic kit producers for applications in microbiology, cell biology, and genetic engineering.

Application

• Chromogenic Substrate in Microbiology: Used in selective and differential culture media (e.g., for E. coli) to detect β-glucuronidase (GUS) activity, producing blue colonies.
• Molecular Biology Research: Employed as a histochemical stain in plant and animal tissues to visualize GUS reporter gene expression in genetic engineering studies.
• Diagnostic Assays: Serves as a key component in enzymatic assays and test kits for the detection and identification of specific bacterial strains.
• Pharmaceutical Quality Control: Utilized in sterility testing and microbial limit tests within pharmaceutical manufacturing environments.
• Food and Water Safety Testing: Applied in methods for the rapid detection of fecal coliforms and specific pathogens in food and water samples.
• Enzyme Activity Studies: Acts as a specific substrate for kinetic studies and inhibition assays of β-glucuronidase enzymes.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-β-D-Glucoside
CAS No.	15548-60-4
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-Gluc; 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside; BCIG; 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranoside; X-Glucuronide; X-β-D-Glucoside; 4-Chloro-5-bromo-3-indolyl-β-D-glucoside
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biological applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Biological Activity	Suitable for enzyme substrate use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.