Description

2-Chloro-4-Nitrophenyl Phosphate is a specialized organic phosphate ester derivative, serving as a critical chromogenic substrate in enzymatic assays. Its primary value lies in its role as a substrate for alkaline phosphatase (ALP), where enzymatic hydrolysis produces a yellow-colored 2-chloro-4-nitrophenolate anion, enabling precise quantitative analysis. This compound is essential for research, diagnostic, and quality control laboratories in the biotechnology, pharmaceutical, and clinical diagnostics industries.

Application

• Diagnostic Reagent: Key chromogenic substrate for measuring alkaline phosphatase (ALP) activity in clinical chemistry analyzers for liver and bone disorder diagnostics.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and the development of colorimetric assay kits for high-throughput screening (HTS).
• Immunoassay Development: Employed in ELISA (Enzyme-Linked Immunosorbent Assay) and other immunoassays where ALP is used as a reporter enzyme.
• Pharmaceutical QC: Utilized in quality control testing of biopharmaceuticals and in-process monitoring of cell culture media.
• Food Safety Testing: Applied in microbial detection assays and food quality testing kits that utilize enzymatic methods.
• Molecular Biology: Serves as a substrate in nucleic acid detection systems coupled with alkaline phosphatase-labeled probes.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl Phosphate
CAS No.	14957-98-3
Molecular Formula	C₆H₄ClNO₆P
Molecular Weight	261.53 g/mol
Synonyms	2-Chloro-4-nitrophenyl phosphate disodium salt; PNPP-2Cl; 2-Chloro-4-nitrophenol phosphate; 4-Nitro-2-chlorophenyl dihydrogen phosphate; Phosphoric acid 2-chloro-4-nitrophenyl ester; ALP Substrate (2-Chloro-4-Nitrophenyl); CNPP; Chromogenic ALP substrate.
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance characteristics, and enzymatic activity validation to ensure optimal performance as a diagnostic substrate. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to guarantee traceability and batch-to-batch consistency for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Keep container tightly sealed when not in use to prevent absorption of atmospheric moisture and CO₂.

Specification

Item	Specification
Appearance	White to off-white crystalline powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	≥ 95% (vs. standard)
Water Content (KF)	≤ 2.0%
Absorbance (λ max)	Conforms (typically ~400-420 nm for hydrolyzed product)
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.