Description

Acrylamido Buffer CAS NO 13276-17-0 is a specialized chemical reagent primarily used as a buffering agent in biochemical and electrophoretic applications. Its value lies in providing a stable pH environment critical for the separation and analysis of proteins and nucleic acids. This product is essential for research laboratories, diagnostic kit manufacturers, and biopharmaceutical companies engaged in gel electrophoresis, chromatography, and molecular biology workflows.

Application

• Polyacrylamide Gel Electrophoresis (PAGE): As a key component in buffer systems for native and SDS-PAGE to separate proteins based on size or charge.
• Western Blotting: Used in transfer buffers for the efficient movement of proteins from gels to membranes.
• Chromatography: Employed as a mobile phase modifier or equilibration buffer in ion-exchange and size-exclusion chromatography of biomolecules.
• Diagnostic Assay Formulation: Provides pH stability in buffers for clinical diagnostic tests and immunoassays.
• Protein Crystallization: Used to establish precise pH conditions in screens for growing protein crystals.
• Molecular Biology Research: Serves as a buffering agent in various enzymatic reactions, DNA/RNA purification, and hybridization procedures.

Basic Information

Product Name	Acrylamido Buffer
CAS No.	13276-17-0
Molecular Formula	C7H13NO4
Molecular Weight	175.18 g/mol
Synonyms	N-(2-Acetamido)-2-aminoethanesulfonic acid; ACES; N-(2-Acetamido)iminodiacetic acid; N-(Carbamoylmethyl)iminodiacetic acid; 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid; ACES Buffer; Good's Buffer ACES; Biological Buffer ACES
EINECS	Contact for details

Quality Control

Our Acrylamido Buffer is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including assay, pH verification, and impurity profiling, to ensure it meets high-purity standards suitable for life science research. Certificates of Analysis (COA) documenting purity, identity, and performance are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (Titration)	≥ 99.0%
pH (1% Solution)	6.7 - 7.1
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
UV Absorbance	Meets research grade criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.