Description

α-Hemolysin is a potent, pore-forming exotoxin primarily produced by *Staphylococcus aureus*, known for its specific lytic activity against mammalian red blood cells. This high-purity biological reagent is critical for research in microbiology, immunology, and cellular biology, where precise and consistent activity is paramount. It is an essential tool for scientists in pharmaceutical R&D, academic institutions, and diagnostic kit manufacturers studying bacterial pathogenesis, host-pathogen interactions, and developing novel therapeutic or detection strategies.

Application

• Biomedical Research: Fundamental studies on bacterial toxin mechanisms, membrane pore formation, and host cell lysis.
• Immunology Assays: Used as a controlled cytolytic agent to study immune cell responses, complement activation, and inflammatory pathways.
• Diagnostic Development: Serves as a key component or positive control in the development of diagnostic assays for *S. aureus* infections and toxin detection.
• Drug Discovery: Employed in high-throughput screening platforms to identify potential inhibitors of hemolysin activity as novel anti-virulence therapeutics.
• Cell Biology Studies: Utilized to create controlled permeability in cell membranes for intracellular delivery studies or to study cell repair mechanisms.
• Vaccine Research: Investigated as a potential antigen or target for vaccine development against staphylococcal diseases.
• Quality Control: Acts as a reference standard in the quality control of related diagnostic reagents and research kits.

Basic Information

Product Name	α-Hemolysin
CAS No.	12616-52-3
Molecular Formula	Contact for details
Molecular Weight	~33 kDa (Monomer)
Synonyms	α-Toxin; Staphylococcal α-hemolysin; α-lysin; Hla; S. aureus α-toxin; α-hemolysin (S. aureus); Pore-forming toxin α-hemolysin
EINECS	Contact for details

Quality Control

Our α-Hemolysin is produced and purified under stringent conditions to ensure high biological activity and lot-to-lot consistency. Each batch is subjected to rigorous quality testing, including assessment of purity, sterility where applicable, and specific hemolytic activity. Certificates of Analysis (COA) detailing purity, potency, and relevant analytical data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C or below to prevent activity loss. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Specific Hemolytic Activity	≥ 1 x 10⁵ HU/mg protein
Endotoxin Level	< 1.0 EU/μg
Solubility	Soluble in aqueous buffers
Identification (Bioassay)	Consistent with standard hemolytic profile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.