Description

Deae Sephadex A-25 is a weak anion exchange chromatography medium based on a cross-linked dextran matrix. This product is essential for the separation and purification of a wide range of biomolecules, including proteins, peptides, and nucleic acids, based on their charge differences. It is a critical tool for researchers and process developers in biopharmaceutical manufacturing, academic research, and diagnostic kit production who require reliable, high-resolution purification.

Application

• Protein and Enzyme Purification: Separation of proteins, including antibodies and enzymes, from complex biological mixtures.
• Nucleic Acid Fractionation: Isolation and purification of DNA, RNA, and oligonucleotides.
• Biopharmaceutical Downstream Processing: Intermediate purification and polishing steps in the manufacturing of therapeutic proteins and vaccines.
• Diagnostic Reagent Preparation: Purification of key components for immunoassays and molecular diagnostic tests.
• Academic and Industrial Research: Laboratory-scale purification for biochemical and molecular biology studies.
• Desalting and Buffer Exchange: Removal of salts and small molecules while changing the buffer environment of biomolecule solutions.

Basic Information

Product Name	Deae Sephadex A-25
CAS No.	12609-80-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DEAE-Sephadex A-25; Diethylaminoethyl Sephadex A-25; Dextran, 2-(diethylamino)ethyl ether, cross-linked; Sephadex, DEAE-; Anion exchanger A-25; Weak anion exchange gel; Chromatography medium, DEAE-dextran; DEAE-Dextran Gel
EINECS	Contact for details

Quality Control

Our Deae Sephadex A-25 is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as ion exchange capacity, particle size distribution, and flow characteristics are verified. Certificates of Analysis (COA) documenting these specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white beads
Ion Exchange Capacity	Contact for details
Particle Size	Contact for details
Swelling Factor	Contact for details
pH Stability Range	2 - 10
Maximum Operating Pressure	Contact for details
Microbial Bioburden	To specification

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.