Description

Azurin CAS NO 12284-43-4 is a blue copper protein isolated from *Pseudomonas aeruginosa*, known for its role in bacterial denitrification and electron transfer. This high-purity biochemical is essential for research in redox biochemistry, enzymology, and microbial metabolism studies. It serves as a critical reagent for academic institutions, biotechnology R&D departments, and pharmaceutical companies investigating bacterial respiration pathways and developing novel antimicrobial strategies.

Application

• Biochemical Research: Used as a model protein for studying long-range electron transfer mechanisms and copper-protein interactions.
• Enzymology Studies: Serves as a substrate or cofactor analog in assays investigating nitrite reductase and other redox enzymes.
• Antimicrobial Research: Investigated for its role in bacterial pathogenesis and as a potential target for novel antibiotic development.
• Biosensor Development: Utilized in the fabrication of electrochemical biosensors due to its stable redox properties.
• Educational Kits: Incorporated into advanced biochemistry and microbiology laboratory teaching kits for protein purification and characterization.
• Structural Biology: Provides a well-characterized protein for X-ray crystallography and NMR spectroscopy studies.

Basic Information

Product Name	Azurin
CAS No.	12284-43-4
Molecular Formula	CnHnNnOnSnCu (Protein)
Molecular Weight	~14,000 Da (Monomer)
Synonyms	Pseudomonas aeruginosa azurin; Bacterial blue copper protein; Copper-containing redox protein; Azurin (Pseudomonas); P. aeruginosa azurin; Electron transfer protein azurin; UNII-9P7K1C6Q4M; Basic blue protein (azurin)
EINECS	Contact for details

Quality Control

Our Azurin is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for purity, copper content, and specific activity. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results from SDS-PAGE, UV-Vis spectroscopy, and activity assays. We adhere to ISO 9001 quality management principles for all production and testing processes.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted solutions should be prepared in appropriate buffers and stored at 2-8°C for short-term use. Avoid repeated freeze-thaw cycles of solutions. Due to its sensitivity, store under inert atmosphere when applicable for extended solution storage.

Specification

Item	Specification
Appearance	Blue lyophilized powder
Identification (UV-Vis)	Characteristic absorption maxima at ~625 nm and ~280 nm
Purity (SDS-PAGE)	≥ 95%
Protein Content (Biuret)	≥ 90%
Copper Content (AAS)	0.9 - 1.1 g-atom per mole protein
Specific Activity	Contact for details
Solubility	Soluble in water and buffer solutions (pH 5-8)
Endotoxin Level	< 1.0 EU/mg (upon request)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.