Description

Succinate Buffer CAS NO 12111-20-5 is a high-purity, aqueous buffer solution formulated from succinic acid and its conjugate base, providing a stable and reliable pH control system in the critical range of approximately 3.8 to 6.0. This product is essential for applications requiring precise and consistent pH maintenance, ensuring the integrity and reproducibility of sensitive biochemical and analytical processes. It is a critical reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries, where controlled ionic strength and pH are paramount.

Application

• Biochemical & Pharmaceutical Research: Used as a buffering agent in enzyme assays, protein purification, cell culture media preparation, and stability studies.
• Chromatography (HPLC/UPLC): Serves as a mobile phase component for the separation and analysis of peptides, proteins, and other biomolecules.
• Diagnostic Kit Manufacturing: Provides a stable pH environment for reagents used in clinical diagnostics and immunoassays.
• Biopharmaceutical Production: Employed in downstream processing, including column chromatography steps for therapeutic protein purification.
• Electrophoresis: Used in capillary electrophoresis (CE) and other electrophoretic techniques as a running buffer.
• Calibration & Standardization: Acts as a pH calibration standard for laboratory instrumentation.

Basic Information

Item	Detail
Product Name	Succinate Buffer
CAS No.	12111-20-5
Molecular Formula	Mixture (C4H6O4 / C4H5O4Na)
Molecular Weight	Mixture
Synonyms	Succinic Acid Buffer; Succinic Acid-Sodium Succinate Buffer; Succinate Buffer Solution; Succinic Acid Disodium Salt Buffer; Butanedioic Acid Buffer; pH Buffer Solution (Succinate); Amber Buffer (Succinate); IUPAC: Butanedioic Acid, Sodium Salt Buffer System
EINECS	Contact for details

Quality Control

Our Succinate Buffer is manufactured under strict quality management systems. Each batch is tested to ensure it meets stringent specifications for pH, conductivity, absorbance, and sterility or bioburden as required. A comprehensive Certificate of Analysis (COA) is provided with every shipment, detailing all test results. We can supply grades suitable for general laboratory use, analytical applications, and GMP-compliant biopharmaceutical manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C). Do not freeze. For long-term stability of certain grades, storage at 2-8°C may be recommended. Refer to the specific product label or COA for detailed storage instructions.

Specification

Item	Specification
Appearance	Clear, colorless solution
pH (at 25°C)	As specified (e.g., 4.0, 5.0, 5.5, 6.0) ± 0.05
Concentration	As specified (e.g., 0.1M, 0.2M)
Conductivity	Within specified range
Absorbance (at 260 nm)	≤ 0.05 AU (for HPLC/UV-sensitive grades)
Absorbance (at 280 nm)	≤ 0.05 AU (for HPLC/UV-sensitive grades)
Filtered / Sterilized	0.22 µm filtered or sterile filtered (as per grade)
Endotoxin	< 0.25 EU/mL (for cell culture/pharma grades)
Bioburden	To specified CFU/mL limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.