Description

α-Actinin is a crucial cytoskeletal protein that plays a fundamental role in cross-linking actin filaments and anchoring them to various cellular structures. This high-purity biochemical is essential for research and development in cell biology, muscle physiology, and disease mechanism studies. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in advanced cellular and molecular research.

Application

• Biomedical Research: A key reagent for studying cytoskeletal architecture, cell adhesion, and motility in normal and diseased states.
• Muscle Physiology Studies: Used to investigate the structure and function of the Z-disc in striated muscle, relevant to cardiomyopathies and muscular dystrophies.
• Diagnostic Development: Serves as a potential antigen or standard in the development of immunoassays for autoimmune or muscle-related disorders.
• Drug Discovery Platforms: Employed in high-content screening assays to identify compounds that modulate cytoskeletal dynamics and cell mechanics.
• Biochemical Assays: Used in pull-down assays, co-immunoprecipitation, and blotting experiments to study protein-protein interactions.
• Cell Culture & Engineering: Applied in research focused on cell signaling pathways linked to the actin cytoskeleton.

Basic Information

Product Name	α-Actinin
CAS No.	11003-00-2
Molecular Formula	Contact for details
Molecular Weight	~100-110 kDa (Isoform dependent)
Synonyms	Actinin Alpha 1; F-Actin Cross-Linking Protein; Non-Muscle α-Actinin 1; ACTN1; Alpha Actinin Cytoskeletal Isoform; α-Actinin-1; FLJ00280
EINECS	Contact for details

Quality Control

Our α-Actinin is produced and purified under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for purity and identity using advanced analytical techniques. A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE), concentration, biological activity, and absence of contaminants is provided with every shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. Thaw on ice or in a refrigerator before use. The product is sensitive to denaturation; avoid exposure to harsh detergents or extreme pH.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Concentration	0.5 - 1.0 mg/mL (Lot specific)
Biological Activity	Confirmed by actin-binding assay
Buffer	Tris or PBS-based buffer, pH 7.0-7.5
Preservative	None
Endotoxin Level	< 1.0 EU/μg (where specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.