Description

2,3-Diphenyl-5-(4-Methoxyphenyl)Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to produce a measurable formazan dye upon reduction, making it indispensable for quantifying cellular metabolic activity and enzymatic function. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors. The product is supplied with a high level of purity and batch-to-batch consistency to ensure reliable and reproducible experimental results.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay Analog): Used to measure mitochondrial activity in live cells as an indicator of cell health, cytotoxicity, and drug efficacy screening.
• Microbiological Testing: Employed in assays to determine microbial viability, growth inhibition, and antimicrobial susceptibility testing.
• Enzyme Activity Detection: Serves as a substrate for dehydrogenase enzymes, enabling the colorimetric detection and quantification of enzymatic reactions in research and diagnostic kits.
• Biochemical Research: Utilized as a redox indicator in various experimental protocols to study electron transfer processes and oxidative stress.
• Pharmaceutical Quality Control: Applied in stability testing and potency assays for biopharmaceutical products.
• Toxicology Studies: A key reagent in in vitro toxicology assessments to evaluate the effects of chemicals on cellular metabolism.

Basic Information

Product Name	2,3-Diphenyl-5-(4-Methoxyphenyl)Tetrazolium Chloride
CAS No.	10560-45-9
Molecular Formula	C26H21ClN4O
Molecular Weight	440.92 g/mol
Synonyms	MTPT; MTT-P; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analog; p-Methoxytetrazolium; 5-(4-Methoxyphenyl)-2,3-diphenyl-2H-tetrazol-3-ium chloride; Tetrazolium Chloride, 2,3-Diphenyl-5-(4-Methoxyphenyl)-; MTT Tetrazolium derivative
EINECS	Contact for details

Quality Control

Every batch of 2,3-Diphenyl-5-(4-Methoxyphenyl)Tetrazolium Chloride is manufactured and tested under a strict quality management system. We ensure the product meets high-purity standards suitable for sensitive biochemical and cell-based applications. Comprehensive analytical testing, including HPLC for purity and identity confirmation, is performed. A Certificate of Analysis (COA) detailing all specifications and test results is provided with each shipment to guarantee traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture. For long-term storage, consider using a desiccator.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.