Description

2,3-Diphenyl-5-(4-Chlorophenyl)Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and a key reagent in biochemical assays. Its primary value lies in its ability to form intensely colored formazan dyes upon reduction, making it indispensable for quantifying cellular metabolic activity and dehydrogenase enzymes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Proliferation Assays (e.g., MTT assay analogs): Used to measure mitochondrial activity in live cells as an indicator of cell health and proliferation.
• Microbiological Testing: Employed as an indicator for bacterial growth and metabolic activity in various microbiological media and diagnostic kits.
• Enzyme Activity Assays: Serves as an electron acceptor in assays for dehydrogenase and reductase enzymes, producing a measurable colorimetric change.
• Histochemistry and Cytochemistry: Utilized as a stain to localize sites of enzymatic activity in tissue sections and cell cultures.
• Pharmaceutical Research: Applied in high-throughput screening (HTS) to evaluate the cytotoxic or cytoprotective effects of drug candidates.
• Food and Environmental Analysis: Used in rapid tests to assess microbial contamination and metabolic activity in samples.

Basic Information

Product Name	2,3-Diphenyl-5-(4-Chlorophenyl)Tetrazolium Chloride
CAS No.	10557-51-4
Molecular Formula	C19H14ClN4•Cl
Molecular Weight	369.25 g/mol
Synonyms	2,3,5-Triphenyl-2H-tetrazolium chloride (4-chloro derivative); (4-Chlorophenyl)diphenyltetrazolium chloride; Tetrazolium Chloride, 2,3-Diphenyl-5-(4-chlorophenyl)-; p-Chlorophenyl-diphenyl-tetrazolium Chloride; TTC (related analog); INT; Iodonitrotetrazolium chloride analog; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-(4-Chlorophenyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.