Description

H-Ala-Onb HBr is a protected amino acid derivative, specifically H-Alanine-O-nitrobenzyl ester hydrobromide, widely used as a critical building block in solid-phase peptide synthesis (SPPS). Its primary value lies in providing a protected alanine residue with a photolabile protecting group, enabling precise, light-directed deprotection for the synthesis of complex peptides and biomolecules. This high-purity intermediate is essential for researchers and manufacturers in pharmaceutical R&D, bioconjugation, and the production of therapeutic peptides and peptidomimetics.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key monomer for the controlled incorporation of alanine using photolytic cleavage conditions.
• Photolithographic Peptide Synthesis: Enabling the spatially addressable, light-directed synthesis of peptide arrays and microchips for high-throughput screening.
• Bioconjugation and Probe Development: Serving as a linker or modified amino acid for creating photo-activatable probes, tags, and peptide-drug conjugates.
• Orthogonal Protection Strategy: Facilitating complex multi-step syntheses where selective deprotection is required alongside other standard protecting groups (e.g., Fmoc, Boc).
• Research & Development: Used in academic and industrial labs for exploring novel peptide structures, bioactive sequences, and material science applications.
• Precursor for Custom Amino Acids: Acting as a starting material for further chemical modification to create non-natural or labeled amino acid derivatives.

Basic Information

Product Name	H-Ala-Onb HBr
CAS No.	10144-66-8
Molecular Formula	C10H13N2O4·HBr
Molecular Weight	305.14 g/mol
Synonyms	H-Ala-ONb HBr; H-Alanine-O-nitrobenzyl ester hydrobromide; Alanine, N-[(phenylmethoxy)carbonyl]-, 4-nitrobenzyl ester, hydrobromide; N-Carbobenzyloxy-alanine 4-nitrobenzyl ester hydrobromide; Z-Ala-ONb HBr; Cbz-Ala-ONb HBr; Benzyloxycarbonyl-alanine 4-nitrobenzyl ester hydrobromide; (S)-2-((((Benzyloxy)carbonyl)amino)propanoic acid 4-nitrobenzyl ester hydrobromide
EINECS	Contact for details

Quality Control

Our H-Ala-Onb HBr is manufactured under strict quality systems to ensure batch-to-batch consistency and high chemical purity suitable for sensitive synthetic applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles. We adhere to cGMP principles where applicable, and our quality protocols are designed to meet the exacting standards of pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent decomposition. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Optical Rotation	Contact for details
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.