Description

p-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Glucopyranoside is a specialized chromogenic substrate widely used in enzymatic assays. This compound is critical for the detection and quantification of N-acetyl-β-D-glucosaminidase (NAGase) activity, a key biomarker for renal and liver function. It is an essential reagent for researchers and manufacturers in the diagnostics, pharmaceutical, and life science sectors requiring precise and reliable enzyme activity measurements.

Application

• Clinical Diagnostics: Primary substrate for colorimetric assays measuring N-acetyl-β-D-glucosaminidase (NAGase) activity in urine and serum, a sensitive indicator for renal tubular damage and liver disorders.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzymes in academic and industrial R&D laboratories.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic agents targeting lysosomal storage diseases or inflammatory conditions.
• Quality Control in Bioprocessing: Monitors enzyme activity and purity during the production of therapeutic proteins and other biologics.
• Veterinary Diagnostics: Applied in animal health testing for early detection of organ-specific injuries.
• Environmental Monitoring: Potential use in assays detecting specific microbial glycosidase activities in environmental samples.

Basic Information

Product Name	p-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Glucopyranoside
CAS No.	10139-02-3
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	4-Nitrophenyl N-acetyl-α-D-glucosaminide; p-Nitrophenyl α-N-acetylglucosaminide; PNP-α-GlcNAc; α-PNP-GlcNAc; NAG Substrate (α-form); 1-O-(4-Nitrophenyl)-2-acetamido-2-deoxy-α-D-glucopyranoside; 2-Acetamido-2-deoxy-α-D-glucopyranosyl 4-nitrophenyl ether
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Glucopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.