Description

Tubercidin 5'-Triphosphate is a chemically modified nucleotide analog where the adenine base is replaced by a 7-deazaadenine (tubercidin) moiety, conjugated to a triphosphate group. This structural modification makes it a valuable substrate and inhibitor for various enzymes involved in nucleic acid metabolism and synthesis. It is primarily utilized in biochemical research and pharmaceutical development for studying RNA polymerases, viral replication mechanisms, and nucleotide-dependent processes. Key industries requiring this high-purity biochemical include academic research institutions, biotechnology companies, and pharmaceutical R&D laboratories focused on enzymology and antiviral drug discovery.

Application

• Enzymology Research: Used as a substrate or competitive inhibitor for studies involving DNA-dependent and RNA-dependent RNA polymerases.
• Antiviral Mechanism Studies: Employed in biochemical assays to investigate the replication mechanisms of viruses and to screen for potential antiviral compounds.
• Nucleotide Metabolism Analysis: A critical tool for probing enzymatic pathways of nucleotide incorporation and metabolism in cellular systems.
• Biochemical Assay Development: Serves as a key component in developing high-throughput screening assays for drug discovery targeting polymerase enzymes.
• Molecular Biology Reagent: Used in in vitro transcription and translation systems to study the effects of modified nucleotides on nucleic acid synthesis and fidelity.
• Pharmaceutical R&D: Acts as a reference standard and a building block in the research and development of novel nucleoside-based therapeutics.

Basic Information

Product Name	Tubercidin 5'-Triphosphate
CAS No.	10058-66-9
Molecular Formula	C11H18N4O13P3
Molecular Weight	507.19 g/mol
Synonyms	7-Deazaadenosine 5'-triphosphate; 7-Deaza-ATP; TTP (ambiguous, use with caution); Tubercidin triphosphate; 4-Amino-5-carbamoyl-7-(β-D-ribofuranosyl)pyrrolo[2,3-d]pyrimidine 5'-triphosphate; 7-Deazaadenosine triphosphate; NSC 280556; 5'-TPP (Tubercidin)
EINECS	Contact for details

Quality Control

Our Tubercidin 5'-Triphosphate is manufactured under stringent quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is analyzed using advanced chromatographic and spectroscopic techniques, including HPLC and NMR, to confirm identity and quantify purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment. We adhere to cGMP guidelines for the production of research-grade biochemicals to support reliable and reproducible scientific outcomes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. For long-term storage, keep desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% (as triphosphate)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.