Description

Naphthol As-Bs Phosphate CAS NO 10019-03-1 is a specialized biochemical reagent primarily used as a substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic activity measurement is required. It is essential for laboratories in the life sciences, clinical diagnostics, and pharmaceutical development sectors seeking reliable and high-purity substrates for colorimetric and fluorometric assays.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Used as a chromogenic substrate for alkaline phosphatase in immunoassays for detecting antibodies or antigens.
• Histochemistry & Cytochemistry: Employed in tissue staining to localize alkaline phosphatase activity in cells and biological samples.
• Clinical Diagnostics: Integral to diagnostic kits for measuring alkaline phosphatase levels, an important biomarker for liver and bone disorders.
• Biochemical Research: Serves as a key reagent in enzymatic studies, kinetics experiments, and inhibitor screening assays in research laboratories.
• Pharmaceutical Quality Control: Utilized in testing procedures within biopharmaceutical manufacturing to ensure product purity and process consistency.
• Molecular Biology: Applied in blotting techniques (e.g., Western, Southern) for the detection of specific proteins or nucleic acids.

Basic Information

Item	Detail
Product Name	Naphthol As-Bs Phosphate
CAS No.	10019-03-1
Molecular Formula	C20H16NO6PS
Molecular Weight	429.38 g/mol
Synonyms	Naphthol AS-BS Phosphoric Acid Ester; 3-Hydroxy-4'-nitro-2-naphthanilide Phosphate; Naphthol AS-BS Phosphate; 4-Nitro-1-naphthol AS-BS Phosphate; Alkaline Phosphatase Substrate; AS-BS Phosphate; Fast Blue RR Salt Coupler; Naphthol AS-BS Acid Phosphate
EINECS	233-017-5

Quality Control

Our Naphthol As-Bs Phosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by IR spectroscopy, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Soluble in organic solvents (e.g., DMF, DMSO)
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.