Description

Fluorescein Di-β-D-Galactopyranoside (FDG) is a fluorogenic substrate specifically designed for the detection of β-galactosidase activity. This compound is critical for high-sensitivity enzymatic assays, where its non-fluorescent form is cleaved by the enzyme to release highly fluorescent fluorescein. It is an essential reagent for researchers and quality control labs in the pharmaceutical, biotechnology, and molecular diagnostics industries, enabling applications from gene expression reporting to cell-based viability assays.

Application

• Reporter Gene Assays: Detection of β-galactosidase (lacZ) gene expression in molecular biology and genetic engineering.
• Cell Viability & Cytotoxicity Testing: Used in assays to measure cell health and the effects of drug candidates or toxic compounds.
• Enzyme Activity Screening: High-throughput screening (HTS) for identifying enzyme inhibitors or activators in drug discovery.
• Microbiology & Diagnostics: Detection of bacterial enzymes (e.g., E. coli) in food safety and clinical diagnostic tests.
• Flow Cytometry & Imaging: Fluorescent labeling of cells expressing β-galactosidase for analysis by flow cytometry or fluorescence microscopy.
• Biochemical Research: Kinetic studies and characterization of β-galactosidase enzyme mechanisms and properties.

Basic Information

Product Name	Fluorescein Di-β-D-Galactopyranoside
CAS No.	1787-20-8
Molecular Formula	C₃₄H₃₄O₁₈
Molecular Weight	730.62 g/mol
Synonyms	FDG; Fluorescein Di-β-D-Galactoside; Fluorescein Digalactoside; 3',6'-Di-O-(β-D-galactopyranosyl)fluorescein; 5(6)-Carboxyfluorescein Di-β-D-Galactopyranoside; β-Galactosidase Substrate FDG; Fluorogenic β-Galactosidase Substrate
EINECS	Contact for details

Quality Control

Every batch of Fluorescein Di-β-D-Galactopyranoside is manufactured under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive research and diagnostic applications. A detailed Certificate of Analysis (COA), including HPLC purity, spectral identification, and impurity profile, is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Background	Low, suitable for high-sensitivity assays
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.