Description

Avidin CAS NO 1405-69-2 is a tetrameric glycoprotein derived from egg white, renowned for its exceptionally high affinity for biotin (vitamin H). This strong, specific, and rapid binding interaction is the cornerstone of its immense value in life science research and diagnostics. It is an indispensable reagent for scientists and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, enabling precise detection, isolation, and purification processes.

Application

• Immunoassay & Diagnostic Kits: Widely used as a detection conjugate in ELISA, lateral flow assays, and other immunoassays due to the biotin-streptavidin/avidin signal amplification system.
• Affinity Purification & Pull-Down Assays: Immobilized on solid supports (e.g., agarose beads) to isolate and purify biotinylated proteins, nucleic acids, or other molecules from complex mixtures.
• Cell & Tissue Staining (Immunohistochemistry/IHC & Flow Cytometry): Serves as a bridge to visualize biotin-labeled antibodies or probes in microscopy and cell analysis.
• Biosensor Development: Utilized to functionalize sensor surfaces with biotinylated capture molecules for detecting specific analytes.
• Drug Delivery & Targeting: Explored in bioconjugation strategies to create targeted therapeutic agents by linking drugs to biotinylated carriers.
• Molecular Biology: Employed in techniques like chromatin immunoprecipitation (ChIP) and fluorescence in situ hybridization (FISH) for capturing biotin-tagged targets.

Basic Information

Product Name	Avidin
CAS No.	1405-69-2
Molecular Formula	Contact for details
Molecular Weight	~68 kDa (tetramer)
Synonyms	Egg White Avidin; Avidin from Egg White; Antibiotin; Vitamin H-binding Protein; Biotin-binding Protein; Glycoprotein avidin; Egg Albumin Avidin; Streptavidin homolog
EINECS	215-783-7

Quality Control

Our Avidin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and performance reliability for critical research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as activity, purity, and endotoxin levels. We adhere to relevant industry standards to support our customers' quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted solutions should be aliquoted and stored at -20°C or below to prevent activity loss. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Protein Content (Biuret)	> 90%
Biotin-Binding Activity	> 10 units/mg protein
Purity (SDS-PAGE)	Single band (~16 kDa subunit)
pH (1% solution)	9.0 - 11.0
Endotoxin (LAL)	< 1.0 EU/mg
Solubility	Soluble in water and neutral buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.