Description

3-Indoxyl-β-D-Glucopyranoside Trihydrate, 99 is a high-purity chromogenic substrate widely used in enzymatic assays and diagnostic applications. This compound serves as a critical reagent for the detection of β-glucosidase activity, producing a distinctive blue color upon enzymatic hydrolysis. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic kit production sectors who require reliable and sensitive substrates for microbiological testing and biochemical research.

Application

• Primary substrate in chromogenic media for the detection and identification of E. coli and other coliform bacteria (e.g., in water and food safety testing).
• Key reagent in diagnostic kits and automated systems for clinical microbiology to differentiate bacterial species based on β-glucosidase activity.
• Biochemical research tool for studying enzyme kinetics, inhibition, and the function of β-glucosidase enzymes in various biological processes.
• Quality control applications in pharmaceutical manufacturing for sterility testing and microbial limit tests.
• Agricultural and environmental microbiology for monitoring microbial populations and enzyme activities in soil and water samples.

Basic Information

Product Name	3-Indoxyl-β-D-Glucopyranoside Trihydrate, 99
CAS No.	1328-73-0
Molecular Formula	C14H17NO7 · 3H2O
Molecular Weight	351.31 g/mol (Anhydrous: 311.29 g/mol)
Synonyms	Indoxyl-β-D-glucoside; 3-Indolyl β-D-glucopyranoside; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide (X-Gluc) related substrate; β-Glucosidase substrate; IBDG; 3-Indoxyl-β-D-glucoside trihydrate; Salicin 3-indoxyl analog; Chromogenic β-glucoside.
EINECS	215-540-0

Quality Control

Our 3-Indoxyl-β-D-Glucopyranoside Trihydrate is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is tested to confirm identity, purity, and absence of interfering impurities. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), water content (Karl Fischer), and microbiological bioburden is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Keep container tightly sealed when not in use to prevent absorption of moisture and CO2.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 99.0%
Water Content (Karl Fischer)	12.0 - 16.0% (Theoretical for trihydrate: ~15.4%)
Specific Rotation [α]D20	-60° to -66° (c=1 in DMF)
Heavy Metals (as Pb)	≤ 10 ppm
Residue on Ignition	≤ 0.1%
Microbial Bioburden	≤ 100 CFU/g
Chromatographic Purity (HPLC)	Single major peak

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.