Description

p-Nitrophenyl 1-Thio-β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection and quantification of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and academic research laboratories for applications in molecular biology, reporter gene assays, and enzyme-linked immunosorbent assays (ELISA).

Application

• β-Galactosidase Assays: As a chromogenic substrate for the colorimetric detection and kinetic analysis of β-galactosidase activity in enzymatic studies.
• Reporter Gene Systems: Used in molecular biology for lacZ reporter gene assays to monitor gene expression and promoter activity.
• Diagnostic Kits: A key component in diagnostic test kits and ELISA formats for detecting specific biomarkers or pathogens.
• Microbiology Research: For studying bacterial gene regulation and identification, particularly in E. coli and other lacZ-containing systems.
• Enzyme Inhibition Studies: Employed in high-throughput screening (HTS) to identify and characterize potential inhibitors of β-galactosidase.
• Cell Biology: Used in histochemical staining to visualize β-galactosidase expression in tissues and cell cultures.

Basic Information

Product Name	p-Nitrophenyl 1-Thio-β-D-Galactopyranoside
CAS No.	1230-27-9
Molecular Formula	C12H15NO7S
Molecular Weight	317.32 g/mol
Synonyms	PNP-β-D-Thiogalactopyranoside; 4-Nitrophenyl 1-Thio-β-D-galactopyranoside; p-Nitrophenyl β-D-Thiogalactoside; 1-Thio-β-D-galactopyranoside 4-nitrophenyl ester; 4-Nitrophenyl β-D-thiogalactopyranoside; PNP-TG; PNPGalS; 4-NPGT
EINECS	214-964-7

Quality Control

Our p-Nitrophenyl 1-Thio-β-D-Galactopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality management standards to guarantee product integrity and supply reliability for our global B2B partners.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.