Description

n-Tosyl-L-Alanine 3-Indoxyl Ester is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of specific proteolytic enzyme activity, particularly in high-throughput screening and diagnostic applications. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent industries for developing sensitive and reliable analytical methods.

Application

• Enzymatic Substrate for Proteases: Acts as a specific chromogenic substrate for enzymes like chymotrypsin, subtilisin, and other serine proteases, producing a visible indigo dye upon cleavage.
• Biochemical Research & Assay Development: Essential tool in academic and industrial R&D for studying enzyme kinetics, inhibition, and mechanism of action.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery platforms to identify and characterize potential protease inhibitors as drug candidates.
• Diagnostic Reagent Manufacturing: Incorporated into kits for the clinical detection and measurement of specific protease levels relevant to various disease states.
• Quality Control in Bioprocessing: Employed to monitor protease activity during the production and purification of biologics and other protein-based therapeutics.
• Microbiology & Cell Biology: Useful in assays designed to detect protease activity secreted by microorganisms or involved in cellular processes.

Basic Information

Product Name	n-Tosyl-L-Alanine 3-Indoxyl Ester
CAS No.	75062-54-3
Molecular Formula	C19H19NO5S
Molecular Weight	373.42 g/mol
Synonyms	L-Alanine, N-[(4-methylphenyl)sulfonyl]-, 2-(1,3-dihydro-3-oxo-2H-indol-2-ylidene) ethyl ester; 3-Indoxyl N-tosyl-L-alaninate; Tosyl-Ala-3-indoxyl ester; TSAIE; N-Tosyl-L-alanine 3-indolyl ester; Protease Substrate (Chymotrypsin); Chromogenic protease substrate; 2-(3-Oxo-1,3-dihydro-2H-indol-2-ylidene)ethyl (2S)-2-[[(4-methylphenyl)sulfonyl]amino]propanoate
EINECS	Contact for details

Quality Control

Our n-Tosyl-L-Alanine 3-Indoxyl Ester is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers' compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This material is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	Off-white to light yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.