Description

Phosphoramidous acid, bis(1-methylethyl)-, (2R,3R,4S)-2-[2-(acetylamino)-6-[[(diphenylamino)carbonyl]oxy]-9H-purin-9-yl]-4-[bis(4-methoxyphenyl)phenylmethoxy]tetrahydro-3-furanyl 2-cyanoethyl ester (9CI) is a sophisticated, high-purity nucleoside phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its precise stereochemistry and protecting groups ensure high coupling efficiency and sequence fidelity, which is paramount for research and therapeutic applications. This compound is essential for pharmaceutical R&D, diagnostic assay development, and academic research focused on antisense oligonucleotides, siRNAs, and aptamers.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated synthesis of DNA and RNA sequences, particularly those requiring modified bases.
• Therapeutic Oligonucleotide Development: Used in the research and production of advanced drug candidates, including antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) for targeted therapies.
• Diagnostic Probe Manufacture: Enables the creation of high-specificity labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH) assays.
• Academic & Biotech Research: A vital reagent for studying nucleic acid chemistry, structure-function relationships, and developing novel bio-conjugates.
• Aptamer and Ribozyme Synthesis: Facilitates the production of complex, functional nucleic acids with catalytic or binding properties for sensor and therapeutic platforms.

Basic Information

Product Name	Phosphoramidous acid, bis(1-methylethyl)-, (2R,3R,4S)-2-[2-(acetylamino)-6-[[(diphenylamino)carbonyl]oxy]-9H-purin-9-yl]-4-[bis(4-methoxyphenyl)phenylmethoxy]tetrahydro-3-furanyl 2-cyanoethyl ester (9CI)
CAS No.	325683-97-4
Molecular Formula	C58H61N7O9P
Molecular Weight	1031.12 g/mol
Synonyms	N6-(Diphenylcarbamoyl)-2'-deoxy-2'-fluoro-5-methyluridine Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-N6-(diphenylcarbamoyl)-2'-deoxy-2'-fluoro-5-methyluridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Me-dFUT Phosphoramidite (DPC); 2'-Deoxy-2'-fluoro-5-methyluridine, N6-diphenylcarbamoyl protected phosphoramidite; 5-Methyl-2'-deoxy-2'-fluorouridine DPC phosphoramidite; 2'-F-5-Me-U Phosphoramidite; Amidite for 2'-F-5-Me-U; 9CI Phosphoramidite Monomer
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal performance in automated synthesizers. Each lot is characterized using advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities like oxidizers or hydrolyzed species. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Assay (NMR)	≥ 97.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.