Description

5'-Dimethoxytrityl-n-p-Isopropylphenoxyacetyl-Guanosine, 2'-o-Tbdms-3'-[(2-Cyanoethyl)-(N,n-Diisopropyl)]-Phosphoramidite is a highly specialized, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of research-grade and therapeutic nucleic acids. It is primarily required by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and primers for diagnostic applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (Guanosine monomer) in automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: For the production of GMP-grade antisense drugs, siRNA, and other nucleic acid therapeutics.
• Research & Diagnostic Probe Synthesis: Used to create labeled and unlabeled probes for PCR, sequencing, and microarray applications.
• Aptamer Development: Enables the synthesis of complex modified DNA/RNA libraries for SELEX processes.
• Synthesis of Modified Oligos: Incorporates the protected guanosine base with specific modifications for stability and binding studies.
• Scale-Up for Pre-clinical & Clinical Trials: Supports the transition from milligram-scale research to gram/kilogram-scale production for drug candidates.

Basic Information

Product Name	5'-Dimethoxytrityl-n-p-Isopropylphenoxyacetyl-Guanosine, 2'-o-Tbdms-3'-[(2-Cyanoethyl)-(N,n-Diisopropyl)]-Phosphoramidite
CAS No.	956580-73-7
Molecular Formula	C58H75N8O9PSi
Molecular Weight	1087.31 g/mol
Synonyms	5'-DMT-N-p-Isopropylphenoxyacetyl-Guanosine, 2'-O-TBDMS-3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]-Phosphoramidite; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-N-[4-(1-methylethoxy)phenyl]acetyl-3'-[(2-cyanoethoxy)(diisopropylamino)phosphinyl]-; dG(iPr-Pac) Phosphoramidite (2'-O-TBDMS); N-p-Isopropyl-phenoxyacetyl Protected dG Phosphoramidite; Fast Deprotecting dG Phosphoramidite; iPr-Pac-dG Phosphoramidite; 956580-73-7; Oligonucleotide Synthesis Monomer (G)
EINECS	Contact for details

Quality Control

Our 5'-Dimethoxytrityl-n-p-Isopropylphenoxyacetyl-Guanosine phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for successful oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity confirmation (by 31P NMR and MS), and moisture content. We adhere to industry-best practices for raw material qualification and in-process controls to support your regulatory filings for therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.