Description

5'-O-DMT-Uridine 3'-CE Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for solid-phase oligonucleotide synthesis. This critical building block enables the precise and automated assembly of RNA and modified oligonucleotide sequences, ensuring high coupling efficiency and yield. It is primarily required by biotechnology, pharmaceutical, and diagnostic companies engaged in the research and development of therapeutic oligonucleotides, antisense drugs, siRNAs, and molecular probes.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated synthesis of RNA strands and oligonucleotides containing uridine.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer-based therapies.
• Research & Diagnostic Probe Development: Synthesis of labeled and unlabeled probes for genomics, PCR, qPCR, and microarray applications.
• Antisense Drug Discovery: Building block for creating modified oligonucleotides with enhanced stability and binding affinity for target mRNA sequences.
• Synthesis of Modified Nucleotides: Intermediate for the preparation of various uridine derivatives with specific functionalizations for advanced research.

Basic Information

Product Name	5'-O-DMT-Uridine 3'-CE Phosphoramidite
CAS No.	951777-44-9
Molecular Formula	C43H55N4O9P
Molecular Weight	802.90 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)uridine 3'-(2-Cyanoethyl N,N-Diisopropylphosphoramidite); DMT-U-CE Phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Uridine Phosphoramidite (DMT protected); U-CE Phosphoramidite; RNA U Phosphoramidite; 5'-DMT-2'-O-TBDMS-Uridine-3'-CE Phosphoramidite (related protected form)
EINECS	Contact for details

Quality Control

Every batch of 5'-O-DMT-Uridine 3'-CE Phosphoramidite is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure it meets the stringent requirements of oligonucleotide synthesis. Specifications typically include purity assessment by HPLC, identity confirmation by NMR and MS, and moisture content analysis. A detailed Certificate of Analysis (COA) is provided with each shipment, guaranteeing traceability and consistency for cGMP and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, the material should be brought to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC, 260 nm)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.