Description

2'-O-[(tert-Butyl)dimethylsilyl]-N-Acetylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. It matters as a critical building block for the precise and efficient incorporation of guanosine into synthetic DNA and RNA sequences, enabling research and therapeutic development. This product is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA research, diagnostic probe development, and other advanced nucleic acid applications.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in automated solid-phase synthesizers for constructing DNA and RNA strands.
• Therapeutic RNA/DNA Development: For the production of antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based therapeutics.
• Diagnostic Probe Manufacture: Used in creating labeled probes for PCR, microarrays, fluorescence in situ hybridization (FISH), and other molecular diagnostics.
• Research-Grade Oligo Production: Essential for academic and industrial research labs synthesizing custom primers, linkers, and gene fragments.
• Synthesis of Modified Oligonucleotides: Enables the introduction of site-specific modifications when used in conjunction with other specialty phosphoramidites.
• Scale-Up and cGMP Manufacturing: Suitable for process development and current Good Manufacturing Practice (cGMP) production of clinical-grade oligonucleotides.

Basic Information

Product Name	2'-O-[(tert-Butyl)dimethylsilyl]-N-Acetylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
CAS No.	944138-03-8
Molecular Formula	C₃₃H₅₀N₇O₇PSi
Molecular Weight	719.86 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-N-Acetyl-Guanosine-3'-CE Phosphoramidite; N2-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG(ib) Phosphoramidite; TBDMS-G(ac) Phosphoramidite; Guanosine, N-[9-[(2R,3R,4R,5R)-5-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy-4-[[(1,1-dimethylethyl)dimethylsilyl]oxy]-3-hydroxy-2-tetrahydrofuranyl]-9H-purin-6-yl]-, acetate (ester); Ac-dG Phosphoramidite (2'-O-TBDMS); G(ac) CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our 2'-O-[(tert-Butyl)dimethylsilyl]-N-Acetylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, typically exceeding 98% (by HPLC). Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications for identity, purity, moisture content, and performance in coupling efficiency tests. We support compliance with relevant guidelines for raw materials used in pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (³¹P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.