Description

4-Azidobutyrate-n-Hydroxysuccinimide Ester is a versatile heterobifunctional crosslinking reagent featuring a photoreactive azide group and an amine-reactive NHS ester. This compound is essential for creating stable covalent bonds between biomolecules, enabling advanced bioconjugation strategies in research and development. It is primarily utilized by scientists and manufacturers in the fields of biopharmaceuticals, proteomics, and materials science for applications such as antibody-drug conjugate (ADC) development, surface functionalization, and protein labeling.

Application

• Bioconjugation and ADC Development: A key reagent for site-specific conjugation of cytotoxic drugs to monoclonal antibodies, forming stable antibody-drug conjugates (ADCs) for targeted cancer therapy.
• Protein and Peptide Labeling: Used to introduce azide handles onto proteins or peptides via the NHS ester, which can subsequently undergo click chemistry reactions for fluorescent or affinity tagging.
• Surface Modification and Immobilization: Functionalizes surfaces (e.g., chips, nanoparticles, beads) with azide groups for the subsequent attachment of biomolecules through bioorthogonal chemistry.
• Polymer and Biomaterial Synthesis: Serves as a crosslinker in the synthesis of smart hydrogels, drug delivery systems, and other functional biomaterials.
• Proteomics Research: Facilitates the study of protein-protein interactions and protein networks through crosslinking mass spectrometry (XL-MS) techniques.
• Diagnostic Assay Development: Employed in the manufacturing of diagnostic kits and biosensors for the stable immobilization of capture antibodies or antigens.

Basic Information

Product Name	4-Azidobutyrate-n-Hydroxysuccinimide Ester
CAS No.	943858-70-6
Molecular Formula	C10H13N5O4
Molecular Weight	267.24 g/mol
Synonyms	4-Azidobutyric Acid N-Hydroxysuccinimide Ester; 4-Azidobutyric Acid Succinimidyl Ester; NHS-Azide; Succinimidyl 4-Azidobutyrate; γ-Azidobutyric Acid N-Hydroxysuccinimide Ester; Azido-NHS Ester; N-(4-Azidobutyryloxy)succinimide; 4-Azido-NHS
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and bioconjugation applications. Each batch is characterized to confirm identity, purity, and functional activity. Certificates of Analysis (COA) detailing specifications such as purity (by HPLC), functional group integrity, and moisture content are available upon request. We adhere to strict in-process controls to guarantee batch-to-batch consistency and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.