Description

6-Azidoriboflavin is a chemically modified derivative of riboflavin (Vitamin B2), where an azido functional group has been introduced, creating a versatile biochemical tool. This compound matters for its role as a photoaffinity labeling agent and a click chemistry handle, enabling precise studies of protein-ligand interactions and biomolecular localization. Researchers and developers in the fields of chemical biology, proteomics, and pharmaceutical R&D need this specialized reagent to investigate flavin-binding proteins and develop targeted diagnostic or therapeutic agents. Its unique structure allows for covalent crosslinking upon photoactivation, facilitating the identification and characterization of biological binding sites.

Application

• Photoaffinity Labeling: Used as a probe to covalently tag and study flavin-dependent enzymes and binding proteins upon UV light exposure.
• Click Chemistry Reagent: Serves as a precursor for bioorthogonal conjugation via azide-alkyne cycloaddition reactions, useful for labeling and imaging.
• Biochemical Research: A critical tool for investigating the mechanism of action, binding sites, and metabolic pathways of riboflavin and its derivatives.
• Proteomics Studies: Employed to identify and map protein targets that interact with flavin cofactors in complex biological systems.
• Pharmaceutical Development: Utilized in the design and synthesis of novel drug candidates that target flavoprotein enzymes.
• Diagnostic Probe Synthesis: Acts as a building block for creating labeled compounds used in diagnostic assays and imaging techniques.

Basic Information

Product Name	6-Azidoriboflavin
CAS No.	101760-83-2
Molecular Formula	C17H20N6O6
Molecular Weight	404.38 g/mol
Synonyms	6-Azido-riboflavin; 6-Azido-7,8-dimethyl-10-((2R,3R,4S)-2,3,4,5-tetrahydroxypentyl)benzo[g]pteridine-2,4(3H,10H)-dione; Vitamin B2 azide derivative; 6-N3-Riboflavin; Azidoflavin; 6-Azido-7,8-dimethyl-10-ribitylisoalloxazine
EINECS	Contact for details

Quality Control

Our 6-Azidoriboflavin is produced and tested under stringent quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. While produced to meet exacting in-house standards, specifications can be aligned with specific research or development requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under low-light conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.