Description

4-Azido-L-Homoalanine, (S)-2-Amino-4-Azidobutanoic Acid Hydrochloride is a specialized, non-canonical amino acid derivative featuring a reactive azido group, making it a valuable click chemistry reagent. This compound is essential for the site-specific modification of peptides and proteins, enabling advanced bioconjugation strategies in drug development and materials science. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and chemical synthesis sectors for creating novel biotherapeutics, diagnostic probes, and functional biomaterials.

Application

• Peptide & Protein Modification: Acts as a bioorthogonal handle for site-specific conjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
• Antibody-Drug Conjugate (ADC) Development: Enables the precise attachment of cytotoxic payloads or fluorescent tags to monoclonal antibodies for targeted cancer therapies and imaging.
• Chemical Biology Probes: Used to incorporate azide functionalities into biomolecules for labeling, pull-down assays, and activity-based protein profiling (ABPP).
• Biomaterial Functionalization: Serves as a building block for synthesizing functionalized polymers, hydrogels, and surfaces with tailored biological activity.
• Solid-Phase Peptide Synthesis (SPPS): Incorporated into custom peptide sequences to introduce unique chemical handles for downstream modifications.
• Metabolic Labeling: Potential use as a methionine analog for studying protein synthesis and turnover in living systems.
• Linker Chemistry: Forms part of cleavable or non-cleavable linkers in prodrug design and controlled-release systems.

Basic Information

Product Name	4-Azido-L-Homoalanine, (S)-2-Amino-4-Azidobutanoic Acid Hydrochloride
CAS No.	942518-29-8
Molecular Formula	C4H8N4O2 • HCl
Molecular Weight	180.59 (Free base) / 217.04 (Hydrochloride)
Synonyms	(S)-2-Amino-4-azidobutanoic acid hydrochloride; L-2-Amino-4-azidobutanoic acid HCl; 4-Azido-L-homoalanine HCl; Azidohomoalanine hydrochloride; AHA HCl; N3-homoAla-OH • HCl; (S)-4-Azido-2-aminobutanoic acid hydrochloride; 4-Azido-L-2-aminobutanoic acid hydrochloride
EINECS	Contact for details

Quality Control

Our 4-Azido-L-Homoalanine Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure enantiomeric purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to guarantee consistency and traceability for your research and development processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool, and well-ventilated area. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the sealed container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Chiral Purity (HPLC)	≥ 98% ee
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.