Description

Bis(2-Cyano-1,1-Dimethylethyl) N,n-Bis(1-Methylethyl)Phosphoramidite is a high-value organophosphorus compound, specifically a phosphoramidite monomer, prized for its role in the synthesis of complex oligonucleotides. Its primary commercial value lies in enabling the precise, automated construction of DNA and RNA sequences, which is fundamental to modern biotechnology and pharmaceutical research. This product is essential for manufacturers and R&D laboratories in the fields of therapeutic oligonucleotides, molecular diagnostics, genetic research, and synthetic biology.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite building block for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for targeted drug therapies.
• Molecular Diagnostic Probes: Synthesis of labeled probes and primers used in PCR, sequencing, and microarray technologies.
• Research-Grade Reagent: For academic and industrial R&D in genomics, molecular biology, and biochemistry.
• Custom Oligo Synthesis Services: A critical raw material for CROs and CDMOs offering custom oligonucleotide production.
• Antisense Technology Development: Enabling research and development of gene silencing and modulation technologies.

Basic Information

Product Name	Bis(2-Cyano-1,1-Dimethylethyl) N,n-Bis(1-Methylethyl)Phosphoramidite
CAS No.	916996-01-5
Molecular Formula	C16H28N3OP
Molecular Weight	309.39 g/mol
Synonyms	Diisopropylamino-bis(2-cyano-1,1-dimethylethoxy)phosphine; 2-Cyanoethyl N,N-Diisopropylphosphoramidite (bis); Bis(2-cyano-1,1-dimethylethyl) diisopropylphosphoramidite; Diisopropylamino-bis(2-cyano-1,1-dimethylethoxy)phosphine; Phosphoramidite monomer; DNA synthesis phosphoramidite; Oligonucleotide synthesis reagent; CE Phosphoramidite (bis)
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as purity, moisture content, and solvent residues. Our quality commitment aligns with the standards expected by the pharmaceutical and biotechnology industries for critical raw materials.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at a recommended temperature of -20°C or below to ensure long-term stability. This product is both hygroscopic (moisture-sensitive) and easily oxidized; therefore, it must be handled in a dry, inert environment and sealed immediately after use to prevent degradation.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.1%
Solvent Residue (GC)	Meets internal specifications
31P NMR	Conforms to reference spectrum

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.