Description

2'-O-Methyl-5-hydroxymethyluridine is a chemically modified nucleoside derivative, a key building block in the synthesis of oligonucleotides. This compound matters for its role in enhancing the stability and efficacy of therapeutic nucleic acids, such as antisense oligonucleotides and siRNA. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and CROs engaged in the development of advanced RNA-based therapeutics and diagnostics.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of modified RNA strands.
• Therapeutic RNA Development: Used to incorporate stability-enhancing modifications into siRNA, antisense oligonucleotides (ASOs), and mRNA for therapeutic applications.
• Research & Diagnostic Probes: Serves as a modified base in the construction of probes for genetic analysis, hybridization, and diagnostic assays.
• Bioconjugation & Labeling: The hydroxymethyl group provides a handle for further chemical conjugation with fluorescent dyes, targeting ligands, or other functional moieties.
• Structural Biology Studies: Employed to study RNA structure, function, and protein-RNA interactions using modified nucleotides.
• Vaccine Platform Development: Investigated for use in next-generation mRNA vaccine platforms to improve stability and translational efficiency.

Basic Information

Product Name	2'-O-Methyl-5-hydroxymethyluridine
CAS No.	910050-95-2
Molecular Formula	C11H16N2O7
Molecular Weight	288.26 g/mol
Synonyms	5-(Hydroxymethyl)-2'-O-methyluridine; 2'-O-Methyl-5-(hydroxymethyl)uridine; 5-Hydroxymethyl-2'-O-methyluridine; 2'-OMe-5-HMU; Uridine, 5-(hydroxymethyl)-2'-O-methyl-; 2'-O-Methyl-5-hydroxymethyl uridine; 2'-OMe-5hmU
EINECS	Contact for details

Quality Control

Our 2'-O-Methyl-5-hydroxymethyluridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and LC-MS for identity verification. We provide comprehensive Certificates of Analysis (COA) with each shipment to ensure traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.