Description

n-Acetyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-Methylguanosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of 2'-O-methylguanosine modifications, which are critical for enhancing the stability, specificity, and therapeutic efficacy of synthetic RNA strands. This product is indispensable for research institutions and pharmaceutical companies engaged in the development of advanced antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based therapeutics and diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: Key building block for automated synthesis of RNA and chimeric oligonucleotides.
• Therapeutic RNA Development: Synthesis of 2'-O-methyl modified guides for siRNA and antisense oligonucleotides (ASOs) to improve nuclease resistance and reduce off-target effects.
• Aptamer and Diagnostic Probe Manufacture: Production of high-affinity nucleic acid ligands and labeled probes for sensing and diagnostic applications.
• Research-Grade Oligo Synthesis: For academic and industrial R&D requiring site-specific 2'-O-methyl modifications to study RNA structure, function, and protein interactions.
• Synthesis of Splice-Switching Oligonucleotides (SSOs): For the development of treatments for genetic disorders by modulating pre-mRNA splicing.
• GMP-Grade Therapeutic Production: Suitable for use in the cGMP-compliant manufacturing of clinical trial and commercial therapeutic oligonucleotides.

Basic Information

Product Name	n-Acetyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-Methylguanosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	909033-40-5
Molecular Formula	C48H55N8O9P
Molecular Weight	919.0 g/mol
Synonyms	2'-O-Methyl-G Phosphoramidite (Ac-DMT); 5'-O-DMT-2'-O-methyl-N2-acetyl-guanosine-3'-CE phosphoramidite; N2-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-methylguanosine-3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; 2'-O-Me-G Phosphoramidite; 2'-O-Methylguanosine phosphoramidite; DMT-2'-O-Me-G(iBu) Phosphoramidite; Guanosine, N-[9-[(2R,3R,4R,5R)-5-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3,4-dihydroxy-4-methyloxolan-2-yl]-6-oxo-1H-purin-2-yl]acetamide, 3-[2-cyanoethoxy(diisopropylamino)phosphino]oxypropanoate (9CI)
EINECS	Contact for details

Quality Control

Every batch of our n-Acetyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-Methylguanosine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is manufactured and tested under strict quality management systems. We ensure high purity and coupling efficiency through comprehensive analytical methods including HPLC, 31P NMR, and 1H NMR. Certificates of Analysis (COA) with detailed specifications are provided for each lot, supporting use in research and cGMP environments. Our quality commitment aligns with the stringent requirements for nucleic acid therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Assay (NMR)	≥ 95.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.