Description

3'(2')-o-(2-Nitro-4-Azidobenzoyl)Adenosine 5'-Triphosphate is a chemically modified nucleotide analog designed for advanced biochemical research and development. This compound is a critical tool for photoaffinity labeling and cross-linking studies, enabling the investigation of ATP-binding sites and enzyme mechanisms. It is primarily utilized by researchers in pharmaceutical discovery, enzymology, and molecular biology who require high-purity, functionalized nucleotides for probing protein-nucleotide interactions.

Application

• Photoaffinity Labeling: Acts as a photoreactive probe to covalently tag and identify ATP-binding proteins and enzymes.
• Enzyme Mechanism Studies: Used to investigate the active sites and catalytic mechanisms of kinases, ATPases, and other nucleotide-dependent enzymes.
• Structural Biology: Aids in the structural analysis of protein complexes by creating irreversible cross-links for analysis via X-ray crystallography or mass spectrometry.
• Drug Discovery Screening: Serves as a key reagent in high-throughput screening assays to identify novel inhibitors of ATP-dependent targets.
• Bioconjugation & Probe Development: Functions as a building block for synthesizing more complex diagnostic or therapeutic probes.
• Nucleic Acid Research: Employed in studies exploring the interactions between nucleotides and nucleic acid polymerases or helicases.

Basic Information

Product Name	3'(2')-o-(2-Nitro-4-Azidobenzoyl)Adenosine 5'-Triphosphate
CAS No.	99308-34-6
Molecular Formula	C22H24N10O18P3
Molecular Weight	857.41 g/mol
Synonyms	2'(3')-O-[2-Nitro-4-azidobenzoyl]adenosine 5'-triphosphate; NAB-ATP; 2-Nitro-4-azidobenzoyl-ATP; 3'-O-(2-Nitro-4-azidobenzoyl)ATP; Photoaffinity ATP analog; caged ATP analog; ATP-NAB; Adenosine-5'-triphosphate, 2'(3')-O-(2-nitro-4-azidobenzoyl)-
EINECS	Contact for details

Quality Control

Our 3'(2')-o-(2-Nitro-4-Azidobenzoyl)Adenosine 5'-Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light will compromise its stability and photoreactive functionality. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (UV/VIS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content	≤ 3.0%
Biological Activity	Functional in standard photo-crosslinking assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.