Description

4-Azidobenzoyl-2-Mercapto-n-Ethylamide-2'-Thiopyridine Disulfide is a specialized heterobifunctional crosslinking agent featuring both a photoactivatable aryl azide group and a cleavable disulfide bond. This compound matters for researchers and manufacturers requiring precise, reversible conjugation of biomolecules, enabling controlled assembly and disassembly in complex systems. It is primarily needed in the fields of bioconjugation, proteomics, and advanced materials science for applications such as affinity labeling, surface immobilization, and the development of stimuli-responsive drug delivery platforms.

Application

• Bioconjugation and Protein Labeling: Used for crosslinking peptides, proteins, or antibodies via its photo-reactive azide group, with the disulfide bond allowing for subsequent reductive cleavage under mild conditions.
• Surface Modification and Immobilization: Facilitates the controlled attachment of biomolecules to gold surfaces or other thiol-reactive substrates for biosensor and microarray development.
• Affinity Chromatography Ligand Synthesis: Serves as a key reagent in the preparation of reversible affinity columns where the target can be eluted by breaking the disulfide linkage.
• Stimuli-Responsive Drug Delivery Systems: Incorporated into nanocarrier designs where the disulfide bond is cleaved in the reducing intracellular environment, triggering payload release.
• Polymer and Materials Science: Acts as a crosslinker in the synthesis of smart polymers and hydrogels with redox-sensitive properties.
• Proteomics Research: Employed in crosslinking mass spectrometry (XL-MS) studies to investigate protein-protein interactions and structural dynamics.

Basic Information

Product Name	4-Azidobenzoyl-2-Mercapto-n-Ethylamide-2'-Thiopyridine Disulfide
CAS No.	97654-07-4
Molecular Formula	C21H18N6O2S3
Molecular Weight	498.60 g/mol
Synonyms	N-[2-[(2-Pyridinyldithio)ethyl]-4-azidobenzamide; 2-Pyridyldithioethyl 4-azidobenzamide; 4-Azidobenzoyl-PDTA; S-(2-((4-Azidobenzoyl)amino)ethyl) 2-pyridyldithioate; 4-Azido-PDTA; Heterobifunctional Crosslinker (Azide/Disulfide); 4-Azidobenzoyl-Pyridyldithioethylamide; 4-Azido-Bz-PDTA
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and development. Each batch is characterized by multiple analytical techniques to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) detailing specific lot results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. Allow the container to reach room temperature in a desiccator before opening to prevent moisture condensation. Store away from strong reducing agents and sources of heat or ignition.

Specification

Item	Specification
Appearance	Light yellow to yellow powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility Test	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.