Description

Thymidylyl(3'-5')Thymidine Cyanoethyl Ester is a protected dinucleoside monophosphate derivative, a crucial synthetic intermediate in oligonucleotide chemistry. Its primary value lies in enabling the controlled, stepwise synthesis of DNA and RNA sequences, particularly thymidine-rich oligomers, with high coupling efficiency and minimal side reactions. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, molecular diagnostics, and genetic research, where precise and scalable nucleic acid synthesis is required.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a key phosphoramidite building block for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Molecular Probe and Primer Synthesis: Creation of labeled and unlabeled probes for PCR, sequencing, and hybridization assays.
• Research & Development: Used in academic and industrial labs for studying nucleic acid structure, function, and for developing novel synthetic methodologies.
• Diagnostic Reagent Production: Manufacture of components for diagnostic kits that rely on specific DNA/RNA sequences.

Basic Information

Product Name	Thymidylyl(3'-5')Thymidine Cyanoethyl Ester
CAS No.	97423-58-0
Molecular Formula	C27H35N4O15P
Molecular Weight	662.56 g/mol
Synonyms	dTpdT-CE; Thymidylyl(3'→5')thymidine 2-cyanoethyl ester; 5'-O-Dimethoxytrityl-thymidylyl(3'-5')-thymidine 3'-(2-cyanoethyl) phosphate; T-T dimer CE; Dinucleoside monophosphate cyanoethyl ester; d(TpT) cyanoethyl ester; Protected T-T dimer; Oligonucleotide synthesis intermediate
EINECS	Contact for details

Quality Control

Our Thymidylyl(3'-5')Thymidine Cyanoethyl Ester is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, critical for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to cGMP principles where applicable to support our customers in pharmaceutical development pipelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under anhydrous conditions in a desiccator. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.