Description

2',3'-o-(2,4,6-Trinitrocyclohexadienylidene)Guanosine 5'-Triphosphate is a chemically modified nucleotide analog, specifically a trinitrophenylated derivative of GTP, designed for advanced biochemical research and diagnostic applications. This compound is valued for its unique chromophoric properties, which enable sensitive detection and analysis in enzymatic and binding assays. It is primarily utilized by researchers in pharmaceutical development, molecular biology, and diagnostic kit manufacturing who require high-purity, specialized nucleotide probes.

Application

• Biochemical Research Probe: Used as a chromogenic substrate or inhibitor in studies of GTP-binding proteins (G-proteins), kinases, and polymerases.
• Diagnostic Assay Development: Serves as a key component in the development of colorimetric or fluorescent diagnostic tests for enzymatic activity.
• Enzyme Kinetics & Screening: Employed in high-throughput screening (HTS) platforms to identify modulators of GTPase activity.
• Nucleic Acid Research: Acts as a modified nucleotide for investigating transcription and translation mechanisms.
• Signal Transduction Studies: Utilized to trace and quantify GTP-dependent signaling pathways in cell biology.

Basic Information

Product Name	2',3'-o-(2,4,6-Trinitrocyclohexadienylidene)Guanosine 5'-Triphosphate
CAS No.	97122-52-6
Molecular Formula	C₁₆H₁₇N₈O₂₀P₃
Molecular Weight	734.27 g/mol
Synonyms	TNP-GTP; 2',3'-O-(2,4,6-Trinitrophenyl)guanosine-5'-triphosphate; Trinitrophenyl-GTP; N²-(2,4,6-Trinitrocyclohexadienylidene)guanosine triphosphate; GTP-ε-TNP; Guanosine-5'-triphosphate, 2',3'-O-(2,4,6-trinitrophenyl)-; TNP-Guanosine triphosphate
EINECS	Contact for details

Quality Control

Every batch of our 2',3'-o-(2,4,6-Trinitrocyclohexadienylidene)Guanosine 5'-Triphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Our quality assurance protocols are designed to meet the stringent requirements of life science research. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Conforms (λmax ~ 340-360 nm)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, aqueous buffer (pH ~7)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.