Description

Thymidine-3'-Phosphoric Acid 4-Nitrophenyl Ester Sodium Salt is a high-purity, activated nucleotide derivative used as a critical reagent in oligonucleotide synthesis and biochemical research. Its core value lies in its role as a phosphoramidite precursor or a substrate for enzymatic studies, enabling the precise construction and modification of DNA sequences. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, molecular diagnostics, and synthetic biology who require reliable, high-quality building blocks for nucleic acid chemistry.

Application

• Oligonucleotide Synthesis: Serves as a key intermediate or activated monomer in solid-phase phosphoramidite chemistry for the automated synthesis of DNA and RNA strands.
• Biochemical Research: Used as a substrate for studying the activity, kinetics, and inhibition of enzymes such as nucleotidyltransferases, polymerases, and phosphatases.
• Probe and Primer Labeling: Facilitates the enzymatic incorporation of labels or modifications into nucleic acid probes for hybridization assays and sequencing.
• Antisense & Therapeutic Oligonucleotide Development: Provides a controlled chemical handle for the site-specific introduction of modifications in drug discovery pipelines.
• Chemical Biology Tool Development: Employed in the synthesis of affinity probes, cross-linkers, or other tools to study nucleic acid-protein interactions.
• Quality Control Standards: Acts as a reference standard for the analysis and quantification of nucleotide-related impurities in pharmaceutical active ingredients.

Basic Information

Product Name	Thymidine-3'-Phosphoric Acid 4-Nitrophenyl Ester Sodium Salt
CAS No.	95648-79-6
Molecular Formula	C16H17N3O9PNa
Molecular Weight	473.29 g/mol
Synonyms	4-Nitrophenyl Thymidine 3'-Monophosphate Sodium Salt; p-Nitrophenyl Thymidine 3'-Phosphate Sodium Salt; Thymidine 3'-(4-Nitrophenyl Phosphate) Sodium Salt; 3'-O-(4-Nitrophenylphosphoryl)thymidine Sodium Salt; T-3'-pNP; PNPP-Thymidine Sodium Salt; Thymidine 3'-Monophosphate p-Nitrophenyl Ester Sodium Salt
EINECS	Contact for details

Quality Control

Our Thymidine-3'-Phosphoric Acid 4-Nitrophenyl Ester Sodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and development applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. A detailed Certificate of Analysis (COA) documenting all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.