Description

Guanosine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-, Disodium Salt is a high-purity nucleotide essential for enzymatic and biochemical research. This compound, commonly known as dGTP-Na₂, serves as a critical building block for DNA synthesis, making it indispensable for molecular biology applications. It is primarily required by biotechnology, pharmaceutical, and diagnostic industries for processes such as PCR, DNA sequencing, and cDNA synthesis.

Application

• Polymerase Chain Reaction (PCR): As one of the four essential deoxynucleoside triphosphates (dNTPs) for DNA amplification.
• DNA Sequencing: Used in Sanger (dideoxy) sequencing and next-generation sequencing (NGS) platforms as a substrate for DNA polymerases.
• cDNA Synthesis: A key component in reverse transcription reactions for generating complementary DNA from RNA templates.
• Site-Directed Mutagenesis: Employed in molecular cloning techniques to introduce specific mutations into DNA sequences.
• DNA Labeling: Can be used in the preparation of radiolabeled or fluorescently labeled DNA probes for hybridization assays.
• Enzymatic Assays: Serves as a substrate for various DNA polymerases, reverse transcriptases, and other nucleic acid-modifying enzymes in kinetic studies.
• Diagnostic Kit Manufacturing: A raw material in the production of kits for genetic testing, pathogen detection, and molecular diagnostics.

Basic Information

Item	Detail
Product Name	Guanosine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-, Disodium Salt
CAS No.	95648-76-3
Molecular Formula	C10H14N5O13P3Na2
Molecular Weight	553.13 g/mol (anhydrous basis)
Synonyms	2'-Deoxyguanosine-5'-triphosphate disodium salt; dGTP-Na₂; Deoxyguanosine triphosphate disodium salt; 2'-Deoxy-GTP disodium; dGTP disodium salt; 2'-Deoxyguanosine 5'-(tetrahydrogen triphosphate) disodium salt; Deoxyguanosine 5'-triphosphate, disodium
EINECS	Contact for details

Quality Control

Our dGTP disodium salt is manufactured under strict quality systems to ensure batch-to-batch consistency suitable for sensitive molecular biology applications. Each lot is tested for identity, purity, and the absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing HPLC purity, pH, endotoxin levels, and bioburden is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 98.0%
pH (1% Solution)	6.5 - 7.5
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Bioburden	< 100 CFU/g
Solubility	Clear, colorless solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.