Description

Difficidin is a naturally occurring macrocyclic lipopeptide antibiotic produced by Bacillus amyloliquefaciens, recognized for its potent and selective activity against Gram-positive bacteria—including multidrug-resistant strains. It matters to global biopharmaceutical and agrochemical developers as a high-value lead compound for novel anti-infective agents and biocontrol solutions. Researchers in antibiotic discovery, veterinary pharmaceuticals, and sustainable crop protection rely on high-purity Difficidin CAS NO 95152-88-8 for mechanistic studies, structure–activity relationship (SAR) profiling, and formulation development.

Application

• Antibiotic drug discovery research targeting resistant Staphylococcus aureus and Enterococcus spp.
• Development of veterinary therapeutics for mastitis and respiratory infections in livestock
• Biological crop protection agent against bacterial blights and wilts in high-value horticulture
• Reference standard for HPLC and LC-MS/MS quantification in regulatory residue analysis
• Tool compound for studying membrane-targeting mechanisms and bacterial cell wall biosynthesis inhibition
• Starting material for semi-synthetic derivatization to improve pharmacokinetic properties
• Quality control standard in GMP-compliant fermentation process monitoring
• Academic and industrial screening libraries for novel antimicrobial scaffolds

Basic Information

Product Name	Difficidin
CAS No.	95152-88-8
Molecular Formula	C63H104N10O15
Molecular Weight	1221.58 g/mol
Synonyms	Difficidin; DifF; Bacillomycin D (misattributed in early literature); Lipopeptide antibiotic B-17; BAC-17; Antibiotic 17; DIF-1; 95152-88-8; Difficidin A; Difficidin B (minor congener)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches are tested per validated methods for identity (IR, HRMS), assay (HPLC-UV), related substances (HPLC), residual solvents (GC), and microbiological purity. The material meets specifications suitable for research and preclinical development under ISO 9001 and cGMP-aligned quality systems.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and avoid repeated exposure to ambient air. Handle under dry nitrogen when weighing.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HRMS)	[M+H]+ m/z 1222.7824 ± 0.005 (C63H106N10O15)
Assay (HPLC-UV)	≥ 95.0%
Related Substances (HPLC)	Single impurity ≤ 1.0%; Total impurities ≤ 3.0%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.