Description

3'-Deoxythymidine 5'-Triphosphate Trilithium Salt is a modified nucleotide triphosphate analog, a critical building block for advanced molecular biology and biochemical research. Its primary value lies in its role as a chain terminator in sequencing applications and as a substrate for studying DNA polymerase mechanisms. This high-purity biochemical is essential for researchers and manufacturers in the fields of molecular diagnostics, enzymology, and next-generation sequencing (NGS) technology development.

Application

• Sanger DNA Sequencing: Used as a dideoxynucleotide (ddTTP) analog for chain termination in traditional sequencing methods.
• Enzymology Studies: Serves as a specific substrate to investigate the kinetics and fidelity of DNA polymerases and reverse transcriptases.
• Molecular Diagnostics: A key component in the development of assays for genetic mutation detection and viral load quantification.
• Nucleic Acid Synthesis: Employed in the enzymatic synthesis of modified oligonucleotides for therapeutic and diagnostic probes.
• Biochemical Research: Fundamental reagent for studying DNA replication, repair, and recombination processes in vitro.
• Antiviral Research: Investigated for its potential as an inhibitor in studies targeting viral polymerases.

Basic Information

Product Name	3'-Deoxythymidine 5'-Triphosphate Trilithium Salt
CAS No.	93939-78-7
Molecular Formula	C10H13Li3N2O13P3
Molecular Weight	508.02 g/mol (anhydrous basis)
Synonyms	2',3'-Dideoxythymidine 5'-triphosphate trilithium salt; ddTTP trilithium salt; 3'-Deoxy-5'-TTP trilithium; Cordycepin triphosphate trilithium salt (related analog); Trilithium 2'-deoxythymidine-5'-triphosphate; ddTTP-Li3; 3'-ddTTP; DNA sequencing terminator
EINECS	Contact for details

Quality Control

Our 3'-Deoxythymidine 5'-Triphosphate Trilithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements for molecular biology-grade reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (anhydrous basis)
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.5 - 8.5
Endotoxin (LAL)	< 10 EU/mg
Filter Sterility	0.2 µm filtered (upon request)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.