Description

Cytidine 5'-(Trihydrogen Diphosphate), 2'-o-Methyl-, Tripotassium Salt is a chemically modified nucleotide analog where the 2'-hydroxyl group of the ribose sugar is methylated. This modification is critical for enhancing the stability and nuclease resistance of oligonucleotides, making it a valuable building block for advanced therapeutic and research applications. It is primarily utilized by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key phosphoramidite precursor for constructing oligonucleotides with enhanced metabolic stability.
• Small Interfering RNA (siRNA) Production: Used to introduce 2'-O-methyl modifications into siRNA strands to improve pharmacokinetic properties and reduce off-target effects.
• Therapeutic Nucleic Acid Development: Essential for R&D in gene silencing, exon skipping, and other RNA-targeting therapies.
• Diagnostic Probe Design: Incorporated into probes for FISH (Fluorescence In Situ Hybridization) and other molecular diagnostics to increase binding affinity and specificity.
• Biochemical Research: Serves as a substrate or inhibitor in enzymatic studies of RNA processing and metabolism.
• Stable Cell Line Generation: Used in the synthesis of modified mRNA for transient protein expression or CRISPR-related applications.

Basic Information

Product Name	Cytidine 5'-(Trihydrogen Diphosphate), 2'-o-Methyl-, Tripotassium Salt
CAS No.	93839-94-2
Molecular Formula	C10H14N3O13P2K3
Molecular Weight	613.46 g/mol
Synonyms	2'-O-Methylcytidine-5'-triphosphate, tripotassium salt; 2'-O-Me-CTP, K3 salt; 2'-O-Methyl-CTP; 5'-CTP, 2'-O-methyl-, tripotassium; Cytidine 5'-(tetrahydrogen triphosphate), 2'-O-methyl-, tripotassium salt; 2'-Methylcytidine triphosphate; N4-Amino-1-(2-O-methyl-β-D-ribofuranosyl)-2-oxo-1,2-dihydropyrimidine 5'-triphosphate tripotassium salt
EINECS	Contact for details

Quality Control

Our production of Cytidine 5'-(Trihydrogen Diphosphate), 2'-o-Methyl-, Tripotassium Salt adheres to stringent quality management protocols suitable for pharmaceutical R&D. Each batch is analyzed to ensure high purity and consistency, with a focus on critical parameters such as HPLC purity, identity confirmation, and residual solvent levels. Certificates of Analysis (COA) detailing comprehensive test results are provided with every shipment to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin	<10 EU/mg
Bioburden	To be specified per grade

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.