Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine is a protected nucleoside derivative, a critical building block in the synthesis of oligonucleotides. This compound matters because it enables the precise and controlled assembly of DNA sequences, particularly those containing inosine bases, which are essential for research in molecular biology and therapeutic development. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic laboratories engaged in antisense therapy, PCR, and genetic diagnostics. Its high purity is paramount for ensuring the fidelity and success of downstream synthetic applications.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for automated DNA/RNA synthesizers.
• Antisense Oligonucleotide (ASO) Development: Used in creating therapeutic oligonucleotides targeting specific mRNA sequences.
• PCR Primer and Probe Synthesis: Enables the incorporation of inosine bases, which act as universal nucleotides, into primers for degenerate PCR.
• Research-Grade Oligo Production: For synthesizing custom DNA strands used in basic molecular biology research, cloning, and sequencing.
• Diagnostic Assay Development: Building block for probes used in genetic testing and in-situ hybridization.
• Synthesis of Modified Nucleosides: Serves as an intermediate for further chemical modification to create novel nucleoside analogs.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine
CAS No.	93778-57-5
Molecular Formula	C31H30N4O6
Molecular Weight	554.59 g/mol
Synonyms	5'-O-DMT-2'-deoxyinosine; DMT-dI; 2'-Deoxyinosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; 5'-O-(Bis(4-methoxyphenyl)phenylmethyl)-2'-deoxyinosine; NSC 339539; 5'-O-(4,4'-Dimethoxytriphenylmethyl)-2'-deoxyinosine; 2'-Deoxyinosine, 5'-[bis(4-methoxyphenyl)phenylmethyl] ether
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyinosine is manufactured under strict quality systems to ensure batch-to-batch consistency suitable for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to GMP principles for production and control, ensuring the material meets the stringent requirements for pharmaceutical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
DMT Content	95 - 102%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.