Description

Pbr322 Plasmid Dna CAS NO 93384-17-9 is a well-characterized, high-copy-number cloning vector, pivotal for molecular biology research and recombinant DNA technology. Its standardized sequence and multiple cloning sites make it an indispensable tool for gene cloning, amplification, and protein expression studies. This product is essential for biotechnology firms, academic research laboratories, and pharmaceutical development units requiring a reliable and versatile plasmid backbone.

Application

• Molecular Cloning: Serves as a standard vector for inserting, amplifying, and manipulating foreign DNA fragments in bacterial hosts like E. coli.
• Gene Expression Studies: Used as a backbone to construct expression vectors for studying gene function and regulation in prokaryotic systems.
• Recombinant Protein Production: Acts as a foundational plasmid for developing systems to produce therapeutic proteins, enzymes, and other biologics.
• Genetic Engineering: A fundamental tool in synthetic biology for constructing genetic circuits and metabolic pathways.
• Academic & Industrial Research: Provides a standardized, well-documented platform for teaching core biotechnological techniques and conducting R&D.
• Quality Control Reference: Used as a positive control in assays for plasmid purification kits, transformation efficiency tests, and sequencing services.

Basic Information

Item	Detail
Product Name	Pbr322 Plasmid Dna
CAS No.	93384-17-9
Molecular Formula	N/A (Biological Macromolecule)
Molecular Weight	Approx. 2.9 x 106 Da (4363 base pairs)
Synonyms	pBR322 DNA; Plasmid pBR322; pBR322 Plasmid; pBR322 Vector; pBR322 Cloning Vector; Escherichia coli R-factor pBR322; ATCC 31344; pBR322 Plasmid Deoxyribonucleic Acid
EINECS	Contact for details

Quality Control

Our Pbr322 Plasmid Dna is produced and purified under stringent conditions to ensure the highest standards of purity and functionality. Each batch undergoes comprehensive analysis, including confirmation of the correct size, supercoiled topology, and absence of contaminating nucleases. A Certificate of Analysis (COA) detailing purity (A260/A280 ratio), concentration, and restriction enzyme digest profile is provided to guarantee batch-to-batch consistency for your critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized or in TE buffer at -20°C. Avoid repeated freeze-thaw cycles of solution-based preparations.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear viscous solution
Concentration	0.5 - 1.0 mg/mL (in solution)
Purity (A260/A280 Ratio)	1.8 - 2.0
Topology	Primarily supercoiled (>90%)
Identification (Restriction Digest)	Yields expected fragment pattern
Endotoxin Level	< 0.1 EU/µg (for transfection-grade)
Biological Activity (Transformation)	> 1 x 108 CFU/µg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.