Description

Poly(Dc).Oligo(Dg)12 CAS NO 125351-09-9 is a defined-sequence oligonucleotide duplex, specifically a poly(dC) strand hybridized with a 12-mer oligo(dG) strand. This synthetic nucleic acid construct is a critical reagent for fundamental biochemical research and diagnostic assay development, offering a stable and predictable model for studying nucleic acid interactions. It is primarily utilized by researchers and developers in the fields of molecular biology, biophysics, and in vitro diagnostics who require high-purity, sequence-specific oligonucleotide complexes.

Application

• Biophysical Studies: Used as a model system for investigating DNA duplex stability, hybridization kinetics, and the effects of modifications or ligands on nucleic acid structure.
• Diagnostic Assay Development: Serves as a positive control, calibration standard, or capture probe component in PCR, qPCR, and other nucleic acid amplification tests (NAATs).
• Enzyme Activity Assays: Acts as a specific substrate for enzymes such as polymerases, nucleases, helicases, and telomerase to study their activity and inhibition.
• Drug Discovery Screening: Employed in high-throughput screening platforms to identify compounds that bind to or interact with G-quadruplex precursors or standard DNA duplexes.
• Quality Control Reference: Provides a standardized material for verifying the performance and specificity of oligonucleotide synthesis and purification processes.
• Teaching and Training: Used in academic and industrial training laboratories as a well-defined nucleic acid sample for experimental protocols.

Basic Information

Product Name	Poly(Dc).Oligo(Dg)12
CAS No.	125351-09-9
Molecular Formula	C120H149N45O77P12 (for one representative chain)
Molecular Weight	~3800 g/mol (for 12-mer, approximate)
Synonyms	Poly(dC)-Oligo(dG)12 Duplex; dC:dG Oligonucleotide Duplex; Polydeoxycytidylic acid - oligodeoxyguanylic acid (12-mer) complex; Oligo(dG)12 / Poly(dC); DNA Duplex (dC:dG)12; Sequence-specific DNA duplex; Synthetic oligonucleotide hybrid; Research oligonucleotide complex
EINECS	Contact for details

Quality Control

Our oligonucleotide products are synthesized under controlled conditions and undergo rigorous analytical testing to ensure sequence fidelity, purity, and performance. Each batch is characterized by HPLC for purity assessment, Mass Spectrometry (MS) for identity confirmation, and UV spectroscopy for accurate quantification. We adhere to ISO 9001 quality management principles. A comprehensive Certificate of Analysis (COA) detailing identity, purity, concentration, and endotoxin levels (where applicable) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For in-use stability, store the prepared stock solutions at 2-8°C for short-term use or aliquot and store at -20°C to -80°C.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (UV)	90% - 110% of stated amount
Oligonucleotide Length	Poly(dC) & 12-mer Oligo(dG)
Extinction Coefficient (ε260)	Provided on COA
Molecular Weight (MS)	Within ± 5 Da of theoretical
Endotoxin (LAL test)	< 10 EU/mg (for molecular biology grade)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.