Description

Dna (Xenopus Tropicalis Clone Ttba021N19 Est (Expressed Sequence Tag)) is a specific, cloned DNA sequence derived from the *Xenopus tropicalis* model organism, identified as an Expressed Sequence Tag (EST). This product is essential for researchers in molecular biology and genomics, providing a validated genetic tool for gene expression studies, functional annotation, and comparative genomics. It is primarily utilized by academic, biotech, and pharmaceutical R&D teams focused on developmental biology, genetic screening, and the construction of genomic libraries.

Application

• Gene Expression Profiling: Used as a hybridization probe or PCR template to study spatial and temporal gene expression patterns in *Xenopus* and related species.
• Functional Genomics: Serves as a key resource for gene function validation through techniques like in situ hybridization, RNA interference (RNAi), or CRISPR-based editing.
• Genomic Library Construction & Screening: Acts as a sequence-verified clone for the assembly of cDNA or genomic libraries, facilitating gene discovery.
• Molecular Cloning & Plasmid Preparation: Provides the source DNA for subcloning into various expression vectors for protein production or further genetic manipulation.
• Comparative and Evolutionary Biology Studies: Enables sequence comparison across species to understand gene conservation, evolution, and function.
• Diagnostic Assay Development: Can be used as a component in developing specific molecular assays or probes for genetic identification.

Basic Information

Product Name	Dna (Xenopus Tropicalis Clone Ttba021N19 Est (Expressed Sequence Tag))
CAS No.	703560-90-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Xenopus tropicalis EST clone Ttba021N19; Clone Ttba021N19 DNA; Expressed Sequence Tag Ttba021N19; Ttba021N19 EST; Xenopus tropicalis cDNA clone Ttba021N19; DNA, Xenopus tropicalis clone Ttba021N19; EST 703560-90-1
EINECS	Contact for details

Quality Control

Our DNA clones are produced and handled under stringent, nuclease-free conditions to ensure sequence integrity and purity. Each batch is verified by restriction enzyme mapping and full insert sequencing to confirm identity and orientation. Certificates of Analysis (COA) detailing concentration, purity (A260/A280 ratio), and verification methods are provided with every shipment to support your research reproducibility and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the lyophilized DNA or DNA solutions at -20°C or below. Avoid repeated freeze-thaw cycles of aqueous solutions. Handle using standard sterile techniques for molecular biology to prevent nuclease contamination.

Specification

Item	Specification
Physical Form	Lyophilized powder or solution in TE buffer
Identification (Sequencing)	Matches reference sequence for clone Ttba021N19
Purity (A260/A280)	1.8 - 2.0
Concentration	As specified on COA (typically 0.1 - 1.0 µg/µL for solutions)
Vector Backbone	Standard cloning vector (e.g., pUC, pBluescript) - Contact for details
Insert Size	As per EST database record - Contact for details
Biological Activity (Functional Test)	Suitable for sequencing, PCR, and restriction digestion
Endotoxin Level	< 0.1 EU/µg (if applicable for sensitive applications)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.