Description

Dna (Rattus Norvegicus Gene Rac Protein Kinase Kinase Cdna Plus Flanks) CAS NO 200678-22-4 is a precisely engineered genetic construct containing the cDNA sequence for the Rac Protein Kinase Kinase gene from Rattus norvegicus (rat), complete with flanking regions for enhanced stability and expression. This material is critical for advanced molecular biology research, enabling the study of Rac signaling pathways, cell motility, and cytoskeletal dynamics. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments focused on oncology, neurology, and developmental biology.

Application

• Gene Function & Pathway Analysis: Used as a template for cloning and expression studies to elucidate the role of Rac Protein Kinase Kinase in cellular signaling networks.
• Recombinant Protein Production: Serves as the genetic material for the expression and purification of the target kinase in various host systems for biochemical assays.
• Cell Biology Research: Essential for transfection experiments to investigate the impact of gene overexpression, knockdown, or mutation on cell morphology, adhesion, and migration.
• Drug Discovery & Target Validation: A key reagent in high-throughput screening platforms and functional assays to identify and characterize novel inhibitors of the Rac signaling pathway.
• Disease Model Development: Used to create stable cell lines or genetically modified models for studying diseases where Rac kinase activity is dysregulated.
• Molecular Probe Generation: Acts as a source for generating labeled probes (e.g., digoxigenin, fluorescence) for in situ hybridization and other detection techniques.

Basic Information

Product Name	Dna (Rattus Norvegicus Gene Rac Protein Kinase Kinase Cdna Plus Flanks)
CAS No.	200678-22-4
Molecular Formula	N/A (Genetic Material)
Molecular Weight	Contact for details
Synonyms	Rac PK Kinase cDNA; Rat Rac Protein Kinase Kinase Gene; p21-Rac1 Activated Kinase cDNA; PAK cDNA (Rattus norvegicus); Rac-PAK Gene Construct; cDNA Clone for Rac-activated kinase; Rat PAK1/2/3 Gene Flanks; Recombinant Rac Kinase Gene Insert
EINECS	Not listed / Not applicable

Quality Control

Every batch of this genetic material is produced and handled under stringent, nuclease-free conditions to ensure integrity and performance. Quality is verified through comprehensive analytical methods including sequence confirmation, purity assessment via agarose gel electrophoresis, and quantification by spectrophotometry. A Certificate of Analysis (COA) detailing batch-specific data such as concentration, A260/A280 ratio, and confirmation of intact flanking regions is provided with each shipment to guarantee research reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot the product upon first use to minimize degradation. Handle using sterile, nuclease-free techniques in a dedicated clean area.

Specification

Item	Specification
Physical Form	Lyophilized powder or solution in TE buffer
Concentration	Contact for details (e.g., 0.1 µg/µL, 1 µg/µL)
Purity (Agarose Gel)	Single, discrete band corresponding to expected size
Purity (A260/A280 Ratio)	1.8 - 2.0
Identification (Sequencing)	Confirms 100% match to reference sequence
Endotoxin Level	< 0.1 EU/µg (if applicable for transfection-grade)
Biological Activity (Functional Test)	Capable of directing expression of active protein in suitable host system

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.