Description

Dna (Human U937 Cell Gene Rac Protein Kinase Kinase Cdna Plus Flanks) is a precisely engineered nucleic acid construct designed for advanced molecular biology and genetic research. This product is critical for scientists studying signal transduction pathways, specifically those involving Rac protein kinase kinase (Rac-PKK) and its role in cellular processes like proliferation, differentiation, and cytoskeletal dynamics. It is an essential tool for researchers in pharmaceutical development, academic institutions, and biotechnology companies focused on oncology, immunology, and neurodegenerative disease research.

Application

• Gene Function and Signaling Pathway Analysis: Used as a template for gene expression studies to elucidate the role of Rac-PKK in U937 cell models.
• Recombinant Protein Production: Serves as the coding sequence for the expression and purification of Rac protein kinase kinase in various host systems.
• Drug Target Validation: Essential for creating cell-based assays to screen and validate potential therapeutic compounds targeting this kinase pathway.
• Molecular Cloning and Plasmid Construction: The cDNA plus flanking sequences provide necessary elements for efficient cloning into expression vectors.
• CRISPR/Cas9 and Gene Editing Studies: Can be used as a reference or donor template for precise genome editing experiments.
• Diagnostic Probe Development: Basis for developing specific probes or primers for detecting gene expression levels in clinical research samples.

Basic Information

Product Name	Dna (Human U937 Cell Gene Rac Protein Kinase Kinase Cdna Plus Flanks)
CAS No.	197829-82-6
Molecular Formula	Nucleic Acid (Custom Sequence)
Molecular Weight	Contact for details (Sequence-dependent)
Synonyms	Rac Protein Kinase Kinase cDNA; Rac-PKK Gene; Human U937 Cell Rac-PKK cDNA; Rac-PKK Expression Construct; Rac (Rho family) Protein Kinase Kinase DNA; p21-Rac1 Activated Kinase Kinase cDNA; PAK Kinase Kinase Gene; Cloning-Ready Rac-PKK Insert
EINECS	Not listed / Not applicable

Quality Control

Our Dna (Human U937 Cell Gene Rac Protein Kinase Kinase Cdna Plus Flanks) is produced and handled under stringent, nuclease-free conditions to ensure integrity and performance. Each batch is verified for sequence accuracy via full-length sequencing and assessed for purity and concentration using spectrophotometric and electrophoretic methods. Certificates of Analysis (COA) detailing these specifications are provided to guarantee the material meets the high standards required for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot the product upon receipt for single-use applications to maintain optimal activity and prevent degradation.

Specification

Item	Specification
Physical Form	Lyophilized powder or solution in TE buffer
Concentration	As specified on COA (typically 0.1-1.0 µg/µL)
Purity (A260/A280 Ratio)	1.8 - 2.0
Sequence Verification	Confirmed by full-length sequencing
Integrity (Gel Electrophoresis)	Single, discrete band of expected size
Endotoxin Level	< 0.1 EU/µg (for transfection-grade material)
Biological Activity (Functional Test)	Confirmed by expression in a suitable host cell system

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.