Description

n-(4-(4-Azido-3-Iodophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysulfosuccinimide Ester is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound matters for its unique dual functionality, combining a photoactivatable aryl azide group with an amine-reactive N-hydroxysulfosuccinimide (NHS) ester, enabling controlled, two-step conjugation strategies under mild conditions. It is primarily needed by researchers and manufacturers in the fields of proteomics, antibody-drug conjugate (ADC) development, and advanced materials science for creating stable, site-specific linkages between biomolecules and various surfaces or probes.

Application

• Bioconjugation and Protein Labeling: Site-specific crosslinking of proteins, antibodies, or peptides to other biomolecules, fluorophores, or solid supports.
• Antibody-Drug Conjugate (ADC) Research: Facilitating the development of novel ADC linkers for targeted cancer therapies.
• Surface Immobilization: Functionalizing biosensor chips, microarray slides, and nanoparticles for diagnostic and research applications.
• Photoaffinity Labeling: Identifying and studying protein-ligand interactions and receptor binding sites using the photoactivatable azide group.
• Polymer and Biomaterial Modification: Introducing reactive handles into hydrogels or polymeric materials for subsequent biomolecule attachment.
• Diagnostic Assay Development: Creating stable conjugates for ELISA, lateral flow assays, and other immunoassay formats.

Basic Information

Product Name	n-(4-(4-Azido-3-Iodophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysulfosuccinimide Ester
CAS No.	93376-02-4
Molecular Formula	C23H20IN7O8S
Molecular Weight	705.41 g/mol
Synonyms	Sulfo-SANPAH; 4-Azido-2,3,5,6-tetrafluorobenzoic acid, N-hydroxysulfosuccinimide ester; N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate (related analog); Heterobifunctional crosslinker, NHS-ester-Azide; NHS-PEG4-Azide (functional analog); Photoactivatable NHS ester; Aryl azide crosslinking reagent; Sulfosuccinimidyl (4-azidosalicylamido) hexanoate (similar structure); Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Every batch of our n-(4-(4-Azido-3-Iodophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysulfosuccinimide Ester is synthesized and purified under controlled conditions to ensure high reactivity and lot-to-lot consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed data on purity, functional group integrity, and solvent content.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Group Assay	≥ 90% active ester by titration
Water Content (KF)	≤ 1.0%
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.