Description

5'-Ethynylthymidine is a chemically modified nucleoside analog, a key building block in medicinal chemistry and life science research. Its unique ethynyl group at the 5' position makes it a versatile intermediate for synthesizing novel antiviral and anticancer agents, particularly through click chemistry applications. This compound is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing next-generation therapeutics and diagnostic probes.

Application

• Pharmaceutical Intermediate: A critical precursor in the synthesis of novel nucleoside-based antiviral and anticancer drugs.
• Click Chemistry Building Block: The terminal alkyne group enables efficient, site-specific conjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) for bioconjugation and probe development.
• Biochemical Research: Used in studies of DNA/RNA synthesis, enzyme inhibition, and metabolic pathways as a modified thymidine analog.
• Probe and Diagnostic Agent Synthesis: Incorporated into fluorescent or radioactive probes for imaging and diagnostic applications.
• Antiviral Research: Serves as a scaffold for developing inhibitors targeting viral polymerases (e.g., HIV, HBV).
• Material Science: Potential use in creating functionalized surfaces or polymers for biosensor applications.

Basic Information

Product Name	5'-Ethynylthymidine
CAS No.	93265-82-8
Molecular Formula	C12H14N2O5
Molecular Weight	266.25 g/mol
Synonyms	5'-Ethynyl-2'-deoxythymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-4-(2-propyn-1-yloxy)pyrimidin-2-one; 2'-Deoxy-5'-ethynylthymidine; 5'-Ethynyl-dT; ETdT; Thymidine, 5'-ethynyl-
EINECS	Contact for details

Quality Control

Our 5'-Ethynylthymidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.