Description

3'-Azido-3'-Deoxythymidine-5'-Triphosphate (AZT-TP) is a modified nucleoside triphosphate analog that serves as a critical biochemical tool for studying DNA synthesis and inhibition. This compound is essential for researchers investigating the mechanisms of antiviral agents, particularly reverse transcriptase inhibitors used in HIV therapy. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and diagnostic kit manufacturers focused on virology and molecular biology.

Application

• Biochemical Research: Used as a substrate analog in enzymatic studies of DNA polymerases and reverse transcriptases to understand inhibition kinetics and mechanisms of action.
• Antiviral Drug Development: Serves as a key reference standard and biochemical probe in the research and development of nucleoside-based antiviral therapeutics.
• Molecular Diagnostics: Employed in the development of assays to screen for compounds that inhibit viral replication cycles.
• Enzyme Kinetics: Acts as a chain terminator in in vitro DNA synthesis reactions to study polymerase fidelity and processivity.
• Metabolic Pathway Studies: Used to trace and analyze the intracellular phosphorylation pathways of prodrug nucleosides like Zidovudine (AZT).
• Quality Control & Reference Standard: Functions as a high-purity analytical standard for pharmaceutical quality control and regulatory compliance testing.

Basic Information

Product Name	3'-Azido-3'-Deoxythymidine-5'-Triphosphate
CAS No.	92586-35-1
Molecular Formula	C10H15N5O13P3
Molecular Weight	506.17 g/mol
Synonyms	AZT-TP; Azidothymidine Triphosphate; 3'-Azido-3'-deoxythymidine 5'-triphosphate; Zidovudine Triphosphate; 3'-Azido-2',3'-dideoxythymidine-5'-triphosphate; 5'-Triphosphoric acid, 3'-azido-3'-deoxythymidine; 2',3'-Dideoxy-3'-azidothymidine-5'-triphosphate; NSC 675186
EINECS	Contact for details

Quality Control

Our 3'-Azido-3'-Deoxythymidine-5'-Triphosphate is manufactured under stringent quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for biochemical reagents, and specifications can be tailored to meet specific research or cGMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.