Description

N4-Benzoyl-5'-o-(Dimethoxytrityl)-7-Deaza-2'-Deoxyadenosine is a high-purity, protected nucleoside derivative essential for oligonucleotide synthesis. This compound is a critical building block for the solid-phase synthesis of modified DNA and RNA strands, enabling advanced research and therapeutic development. It is primarily used by pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on antisense oligonucleotides, aptamers, and other nucleic acid-based technologies.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated solid-phase synthesis of DNA and RNA strands containing 7-deaza-2'-deoxyadenosine modifications.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications targeting genetic diseases.
• Aptamer Production: Enables the creation of modified DNA aptamers with enhanced binding affinity and nuclease resistance for diagnostic and therapeutic use.
• PCR Probe & Primer Design: Incorporation into probes and primers to improve thermal stability and reduce non-specific binding in molecular diagnostics.
• Structural Biology Studies: Used to synthesize modified nucleic acids for studying DNA/RNA structure, function, and protein interactions.
• Next-Generation Sequencing (NGS): Can be utilized in the development of novel sequencing technologies and modified nucleotide libraries.

Basic Information

Product Name	N4-Benzoyl-5'-o-(Dimethoxytrityl)-7-Deaza-2'-Deoxyadenosine
CAS No.	90335-57-2
Molecular Formula	C43H37N5O6
Molecular Weight	719.79 g/mol
Synonyms	7-Deaza-2'-deoxyadenosine, N4-benzoyl-5'-O-(4,4'-dimethoxytrityl)-; 5'-O-DMT-N4-benzoyl-7-deaza-2'-deoxyadenosine; N4-Benzoyl-5'-O-DMT-7-deaza-2'-deoxyadenosine; 7-Deaza-dA(Bz) Phosphoramidite precursor; 2'-Deoxy-7-deazadenosine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; 4-[(2R,3S,5R)-5-[4-(Benzoylamino)-1H-pyrrolo[2,3-d]pyrimidin-7-yl]-2-(hydroxymethyl)oxolan-3-yl]oxy-4',4''-dimethoxytrityl alcohol (protected form).
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-5'-o-(Dimethoxytrityl)-7-Deaza-2'-Deoxyadenosine is manufactured under strict quality systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed under an inert atmosphere (argon or nitrogen) when not in use to prevent degradation. For long-term storage, consider storing at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.